Abstract
In this study we characterized the subtypes of nucleotide P2Y receptors that respond to ADP in glioma C6 cells. Direct visualization of phosphatidylinositol 4,5-bisphosphate at the cell surface revealed that extracellular ADP activates phospholipase C (PLC). Knock-down of P2Y(1) receptor with antisense oligonucleotide, as well as treatment with MRS2179 and pyridoxal-phosphate-6-azophenyl-2',4'-disulfonic acid (P2Y(1) antagonists), attenuates receptor-mediated PLC activity. Adenylyl cyclase inhibition by ADP remains unchanged under these conditions. Reverse transcription-PCR analysis showed that P2Y(12) receptor is expressed in C6 cells. We therefore conclude that, in glioma C6 cells, two P2Y receptor subtypes are present: P2Y(1), coupled to PLC, and P2Y(12), negatively coupled to adenylyl cyclase.
References
Nov 15, 1995·The Biochemical Journal·M J Berridge
Aug 1, 1995·British Journal of Pharmacology·E FeoldeC Frelin
Mar 1, 1995·Cell Calcium·J BarańskaP Sabała
Jul 17, 1995·Biochemical and Biophysical Research Communications·D J HendersonI A Dainty
Jun 6, 1995·Biochemical and Biophysical Research Communications·Y TokuyamaG I Bell
Oct 1, 1994·British Journal of Pharmacology·J L BoyerT K Harden
Oct 1, 1993·Endocrine Reviews·J W Putney, G S Bird
Jun 14, 1993·FEBS Letters·T E WebbE A Barnard
Jun 1, 1996·Gene·C LéonC Gachet
May 1, 1996·British Journal of Pharmacology·J B SchachterT K Harden
Dec 24, 1997·British Journal of Pharmacology·J B SchachterT K Harden
May 19, 1998·Trends in Pharmacological Sciences·M R Boarder, S M Hourani
Oct 24, 1998·The Journal of Cell Biology·P Várnai, T Balla
Feb 13, 2001·British Journal of Pharmacology·P SabalaJ Barańska
Feb 22, 2001·Methods : a Companion to Methods in Enzymology·B F BakerB P Monia
Feb 24, 2001·Nature·G HollopeterP B Conley
May 12, 2001·Il Farmaco·K A JacobsonJ L Boyer
Jun 16, 2001·British Journal of Pharmacology·J JinS P Kunapuli
Oct 2, 2001·Journal of Neurochemistry·B GrobbenH Slegers
Citations
Apr 12, 2008·Purinergic Signalling·Luz María G CarrasqueroM Teresa Miras-Portugal
Apr 12, 2008·Purinergic Signalling·Dorota SuplatJolanta Barańska
Nov 26, 2010·Purinergic Signalling·Dorota Supłat-WypychJolanta Barańska
Apr 25, 2012·Purinergic Signalling·Gary A WeismanLaurie Erb
Jun 26, 2013·Purinergic Signalling·Geoffrey Burnstock, Francesco Di Virgilio
Jun 26, 2008·Pathology Oncology Research : POR·Tamás Deli, László Csernoch
May 26, 2005·Biochemical Pharmacology·Yong-Chul KimKenneth A Jacobson
May 20, 2005·Proceedings of the National Academy of Sciences of the United States of America·Sami R FamRandy A Hall
Apr 12, 2008·Purinergic Signalling·Kristof Van Kolen, Herman Slegers
Oct 31, 2003·Journal of Medicinal Chemistry·Hak Sung KimKenneth A Jacobson
Feb 13, 2007·Brain Research Bulletin·Patryk KrzeminskiJolanta Brańska
Apr 21, 2006·The FEBS Journal·Kristof Van Kolen, Herman Slegers
Mar 16, 2007·The FEBS Journal·Patryk KrzemińskiJolanta Barańska
Mar 26, 2004·Biochemical Pharmacology·Patrik ClaesHerman Slegers
Feb 21, 2006·European Journal of Pharmacology·Edgar Julian Paredes-GameroAlice Teixeira Ferreira
Jan 14, 2004·British Journal of Pharmacology·Rafal CzajkowskiJolanta Baranska
Feb 20, 2018·Frontiers in Pharmacology·Patrizia BalleriniPaola Patrignani
Dec 12, 2007·Journal of Cellular Biochemistry·Ju-Yun WengSynthia H Sun
Feb 26, 2020·International Journal of Molecular Sciences·Alexandre MansourIsabelle Gouin-Thibault
Aug 2, 2018·Cancers·Annalisa BrunoPaola Patrignani
Aug 7, 2002·Biochemical and Biophysical Research Communications·Pawelz SabałaJolanta Barańska
Aug 6, 2005·Neurochemistry International·Katrin Sak, Peter Illes
Mar 15, 2006·Trends in Pharmacological Sciences·Nicholas White, Geoffrey Burnstock
Sep 20, 2007·European Journal of Pharmacology·Satoko OhkuboNorimichi Nakahata
Jul 22, 2008·European Journal of Pharmacology·Patryk KrzemińskiJolanta Barańska