PMID: 9182710Jun 1, 1997Paper

ADP-glucose drives starch synthesis in isolated maize endosperm amyloplasts: characterization of starch synthesis and transport properties across the amyloplast envelope

The Biochemical Journal
T MöhlmannH E Neuhaus

Abstract

We recently developed a method of purifying amyloplasts from developing maize (Zea mays L.) endosperm tissue [Neuhaus, Thom, Batz and Scheibe (1993) Biochem. J. 296, 395-401]. In the present paper we analyse how glucose 6-phosphate (Glc6P) and other phosphorylated compounds enter the plastid compartment. Using a proteoliposome system in which the plastid envelope membrane proteins are functionally reconstituted, we demonstrate that this type of plastid is able to transport [14C]Glc6P or [32P]Pi in counter exchange with Pi, Glc6P, dihydroxyacetone phosphate and phosphoenolpyruvate. Glucose 1-phosphate, fructose 6-phosphate and ribose 5-phosphate do not act as substrates for counter exchange. Besides hexose phosphates, ADP-glucose (ADPGlc) also acts as a substrate for starch synthesis in isolated maize endosperm amyloplasts. This process exhibits saturation kinetics with increasing concentrations of exogenously supplied [14C]ADPGlc, reaching a maximum at 2mM. Ultrasonication of isolated amyloplasts greatly reduces the rate of ADPGlc-dependent starch synthesis, indicating that the process is dependent on the intactness of the organelles. The plastid ATP/ADP transporter is not responsible for ADPGlc uptake. Data are presented that ...Continue Reading

Citations

Mar 19, 2005·The Plant Journal : for Cell and Molecular Biology·Hardy RolletschekLjudmilla Borisjuk
Mar 12, 2004·Annual Review of Plant Physiology and Plant Molecular Biology·H. E. Neuhaus, M. J. Emes
Jul 20, 2018·Planta·Henrique NoronhaHernâni Gerós
Mar 2, 2005·The Journal of Biological Chemistry·Michaela LerochJoachim Tjaden
Jun 15, 2007·The Journal of Biological Chemistry·Simon KirchbergerJoachim Tjaden
Jun 29, 2001·Proceedings. Biological Sciences·R ViolaM Pedersén
Jun 10, 2009·Journal of Experimental Botany·Sylviane Comparot-Moss, Kay Denyer
Aug 30, 2018·Nature Communications·Marie-Christine KleinIlka Haferkamp
Jun 1, 2007·Functional Plant Biology : FPB·Thomas Martin, Frank Ludewig
Feb 10, 1999·Plant Physiology·B Wischmann Lindberg Moller B
May 20, 2003·Current Opinion in Plant Biology·Martha G JamesAlan M Myers
Mar 6, 1999·Current Opinion in Plant Biology·U I Flügge

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