PMID: 8460266Mar 1, 1993Paper

ADP induces desensitisation of equine platelet aggregation responses: studies using ADP beta S, a stable analogue of ADP

Research in Veterinary Science
Alastair W PooleR J Evans

Abstract

Pre-incubation of equine platelets in platelet-rich plasma with adenosine 5'-diphosphate (ADP) induced a reduction in aggregation responsiveness to subsequent additions of ADP. The desensitisation was shown to be homologous since the responsiveness to platelet-activating factor, thrombin, collagen, 5-hydroxytryptamine or ionomycin remained unchanged. Adenosine 5'-(beta-thio)-diphosphate (ADP beta S), a non-hydrolysable analogue of ADP, was shown to act as an agonist inducing aggregation by interaction with the ADP receptor. ADP beta S was then used in the desensitisation studies in which residual ADP was degraded by the addition of apyrase. The desensitisation to ADP beta S fully recovered by one hour after pre-treatment with ADP and was not induced by an extracellular mediator. The mechanism of desensitisation is therefore likely to involve the ADP receptor or proximal intermediates in the signal transduction pathway for ADP.

References

Sep 3, 1977·The Veterinary Record·J C Greatorex
Aug 1, 1975·Proceedings of the National Academy of Sciences of the United States of America·M HambergB Samuelsson
Jan 1, 1990·Annals of the New York Academy of Sciences·N J Cusack, S M Hourani
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Jan 1, 1986·Methods in Enzymology·A LundinA Pousette
Jun 1, 1981·British Journal of Pharmacology·N J Cusack, S M Hourani

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Citations

Nov 14, 1996·European Journal of Pharmacology·G E JarvisM F Heath
Jun 3, 2008·Journal of Thrombosis and Haemostasis : JTH·S J MundellA W Poole
Feb 10, 2012·Journal of Veterinary Emergency and Critical Care·Robert Goggs, Alastair W Poole
Dec 20, 2007·Journal of Thrombosis and Haemostasis : JTH·J F BartonS J Mundell
Jun 29, 2006·Molecular Pharmacology·Stuart J MundellAlastair W Poole

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