PMID: 2492153Jan 1, 1989Paper

ADP release from myosin in permeabilized smooth muscle

The American Journal of Physiology
T M ButlerM J Siegman

Abstract

The purpose of this study was to determine the nucleotide bound to myosin and its rate of release under relaxed and activated conditions in permeabilized rabbit portal veins. Incubation of the muscles in a relaxing solution containing [3H]-ATP resulted in the formation of 60-70 microM radiolabeled ADP in the muscle whether or not the myosin light chains had been thiophosphorylated. This value was similar to the estimate of the concentration of myosin subfragment 1. Upon transfer of the muscles to a chase solution containing no labeled ATP, there was a very slow loss of labeled ADP when the light chains were unphosphorylated, but a much faster release occurred when the light chains were thiophosphorylated. The results suggest that smooth muscle myosin exists primarily in a complex with ADP under both relaxed and phosphorylated conditions and that phosphorylation of all of the light chains results in a large increase in the rate of release of the products of ATP splitting from all of the myosin. Interestingly, the exponential release of ADP in relaxed muscle shows two components, one of which contains about two-thirds of the total ADP and is 5- to 10-fold faster than the other. If the difference in rates of ADP release observed i...Continue Reading

References

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Citations

Apr 1, 1994·Molecular and Cellular Biochemistry·J F Clark
Aug 1, 1994·Journal of Muscle Research and Cell Motility·J F ClarkR Ventura-Clapier
May 20, 1992·Biochimica Et Biophysica Acta·J F ClarkR Ventura-Clapier

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