ADP-ribosylating and vacuolating cytotoxin of Mycoplasma pneumoniae represents unique virulence determinant among bacterial pathogens.

Proceedings of the National Academy of Sciences of the United States of America
Thirumalai R Kannan, Joel B Baseman

Abstract

Unlike many bacterial pathogens, Mycoplasma pneumoniae is not known to produce classical toxins, and precisely how M. pneumoniae injures the respiratory epithelium has remained a mystery for >50 years. Here, we report the identification of a virulence factor (MPN372) possibly responsible for airway cellular damage and other sequelae associated with M. pneumoniae infections in humans. We show that M. pneumoniae MPN372 encodes a 68-kDa protein that possesses ADP-ribosyltransferase (ART) activity. Within its N terminus, MPN372 contains key amino acids associated with NAD binding and ADP-ribosylating activity, similar to pertussis toxin (PTX) S1 subunit (PTX-S1). Interestingly, MPN372 ADP ribosylates both identical and distinct mammalian proteins when compared with PTX-S1. Remarkably, MPN372 elicits extensive vacuolization and ultimate cell death of mammalian cells, including distinct and progressive patterns of cytopathology in tracheal rings in organ culture that had been previously ascribed to infection with WT virulent M. pneumoniae. We observed dramatic seroconversion to MPN372 in patients diagnosed with M. pneumoniae-associated pneumonia, indicating that this toxin is synthesized in vivo and possesses highly immunogenic epito...Continue Reading

References

Jan 1, 1976·Infection·M Majer
Jun 1, 1975·Proceedings of the National Academy of Sciences of the United States of America·B H Iglewski, D Kabat
Mar 1, 1969·The Journal of Infectious Diseases·G W Fernald
Dec 1, 1969·Proceedings of the Society for Experimental Biology and Medicine·A M CollierF W Denny
Jun 1, 1984·Proceedings of the National Academy of Sciences of the United States of America·S F Carroll, R J Collier
Jan 1, 1980·Virchows Archiv. A, Pathological Anatomy and Histology·G F MurphyJ E Craighead
Sep 1, 1982·Journal of Bacteriology·J B BasemanD K Leith
Jan 1, 1995·Clinical Microbiology Reviews·K M Krueger, J T Barbieri
Oct 11, 1994·Proceedings of the National Academy of Sciences of the United States of America·E PapiniC Montecucco
Jan 1, 1993·Sub-cellular Biochemistry·J B Baseman
Aug 1, 1995·Microbial Pathogenesis·J B BasemanC A Thomas
Apr 1, 1996·Molecular Microbiology·D C Krause
Jul 1, 1996·Molecular Microbiology·T E Colyer, N M Kredich
Oct 1, 1996·American Journal of Respiratory and Critical Care Medicine·J B BasemanS F Dallo
Jan 1, 1997·Emerging Infectious Diseases·J B Baseman, J G Tully
Oct 14, 2000·Microbial Pathogenesis·S F Dallo, J B Baseman
Jan 10, 2001·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·K R Khubchandani, J M Snyder
Feb 22, 2001·Annual Review of Physiology·E Crouch, J R Wright
Oct 19, 2004·Clinical Microbiology Reviews·Ken B Waites, Deborah F Talkington

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Citations

Oct 5, 2013·Current Allergy and Asthma Reports·T Prescott Atkinson
Nov 5, 2011·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·T R KannanJ B Baseman
Oct 1, 2011·The Journal of Infectious Diseases·T R KannanJoel B Baseman
Dec 9, 2008·Molecular Plant-microbe Interactions : MPMI·Xiaodong BaiSaskia A Hogenhout
Mar 9, 2010·Acta Crystallographica. Section F, Structural Biology and Crystallization Communications·Olga N PakhomovaP John Hart
Jun 15, 2010·Applied and Environmental Microbiology·Radha KrishnakumarJohn I Glass
Aug 31, 2010·Applied and Environmental Microbiology·Ayman B AllamMary B Brown
Jul 8, 2011·Clinical Microbiology Reviews·David Taylor-Robinson, Jørgen Skov Jensen
Aug 5, 2009·Infection and Immunity·Coreen JohnsonJoel B Baseman
Apr 16, 2008·Infection and Immunity·Sowmya BalasubramanianJoel B Baseman
Sep 10, 2008·Infection and Immunity·T R KannanJoel B Baseman
Feb 4, 2010·Infection and Immunity·Eric BaranowskiChristine Citti
Jan 9, 2013·Infection and Immunity·Stephanie GroßhennigJörg Stülke
Jul 11, 2008·Journal of Clinical Microbiology·Jonas M WinchellBarry S Fields
Dec 17, 2008·Journal of Clinical Microbiology·Roger Dumke, Enno Jacobs
Jan 8, 2010·Cellular Physiology and Biochemistry : International Journal of Experimental Cellular Physiology, Biochemistry, and Pharmacology·Zissis C ChroneosVirginia L Shepherd
May 29, 2010·American Journal of Respiratory and Critical Care Medicine·Chonnamet TechasaensiriR Doug Hardy
Jan 28, 2012·American Journal of Respiratory Cell and Molecular Biology·Jorge L MedinaPeter H Dube
Dec 21, 2010·PLoS Computational Biology·Robert J FieldhouseA Rod Merrill
Dec 17, 2008·Future Microbiology·Ken B WaitesT Prescott Atkinson
Feb 10, 2016·Frontiers in Microbiology·Roger Dumke, Enno Jacobs
Apr 8, 2015·Proceedings of the National Academy of Sciences of the United States of America·Argentina BeckerP John Hart
Apr 30, 2013·Annals of Allergy, Asthma & Immunology : Official Publication of the American College of Allergy, Asthma, & Immunology·Pamela R WoodEdward G Brooks
Jan 19, 2016·Indian Journal of Medical Microbiology·R ChaudhryA Chandolia
May 1, 2012·Enfermedades infecciosas y microbiología clínica·María Antonia Meseguer-PeinadoGemma Codina-Grau
Mar 5, 2016·Frontiers in Microbiology·Mitchell F Balish, Steven L Distelhorst
Sep 26, 2008·Trends in Biochemical Sciences·Robert J Fieldhouse, A Rod Merrill
Sep 1, 2015·International Journal of Medical Microbiology : IJMM·Enno JacobsRoger Dumke
Jul 30, 2009·Liver International : Official Journal of the International Association for the Study of the Liver·Christoph P BergGerburg M Stein

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