PMID: 2105501Feb 1, 1990Paper

ADP-ribosylation factor is functionally and physically associated with the Golgi complex

Proceedings of the National Academy of Sciences of the United States of America
T StearnsR A Kahn

Abstract

ADP-ribosylation factor (ARF) is a ubiquitous, highly conserved 21-kDa GTP-binding protein, first identified in animal cells as the cofactor required for the in vitro ADP-ribosylation of the stimulatory regulatory subunit of adenylate cyclase, Gs, by cholera toxin. As the relevance of this activity to in vivo function is unknown, we have taken advantage of the conserved nature of ARF to study its function in Saccharomyces cerevisiae. Yeast cells bearing an arf1 null mutation display a number of phenotypes suggesting a defect in the secretory pathway. Secreted invertase is only partially glycosylated, and there is a small internal accumulation of invertase. Genetic experiments revealed interactions between ARF1 and other genes known to be involved in the secretory pathway, including YPT1, which encodes a different GTP-binding protein. In accord with these genetic results, immunofluorescence and immunoelectron microscopy show that ARF protein is localized to the Golgi apparatus in mammalian cells, in particular to the cytosolic surface of predominantly cis-Golgi membranes. Together, these results indicate that ARF functions in intracellular protein transport to or within the Golgi apparatus, a role not predicted by the previous i...Continue Reading

References

Jul 1, 1988·Proceedings of the National Academy of Sciences of the United States of America·J L Sewell, R A Kahn
Jan 1, 1987·Annual Review of Biochemistry·M Barbacid
Jul 1, 1973·Proceedings of the National Academy of Sciences of the United States of America·J Jarvik, D Botstein
Aug 1, 1981·Cell·P NovickR Schekman

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Citations

Oct 1, 1990·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·A P Newman, S Ferro-Novick
Mar 1, 1995·Medicinal Research Reviews·G PowisK W Sherrill
May 24, 1996·Chemistry and Physics of Lipids·S Cockcroft
Jul 10, 1991·Biochimica Et Biophysica Acta·S Nishikawa, A Nakano
Sep 1, 1996·Journal of Lipid Mediators and Cell Signalling·R A KahnP A Randazzo
Aug 1, 1991·Current Opinion in Cell Biology·B Goud, M McCaffrey
Aug 1, 1992·Current Opinion in Cell Biology·R Schekman
Aug 1, 1994·Current Opinion in Cell Biology·J G Donaldson, R D Klausner
Aug 1, 1995·Current Opinion in Cell Biology·N R Salama, R W Schekman
Apr 1, 1992·Trends in Cell Biology·F A BarrW B Huttner
Jul 9, 1999·Progress in Lipid Research·J C DeMarR E Anderson
Aug 1, 1997·Molecular and Biochemical Parasitology·F J LeeC Syin
Dec 11, 1996·Biochimica Et Biophysica Acta·O SaitohH Nakata
Feb 13, 2001·Plant Science : an International Journal of Experimental Plant Biology·A Kobayashi-UeharaH Handa
Apr 25, 2006·Nature Reviews. Molecular Cell Biology·Crislyn D'Souza-Schorey, Philippe Chavrier
Apr 15, 1991·Proceedings of the National Academy of Sciences of the United States of America·J W TamkunJ A Kennison
Jul 15, 1992·Proceedings of the National Academy of Sciences of the United States of America·J G DonaldsonR D Klausner
Jul 15, 1992·Proceedings of the National Academy of Sciences of the United States of America·S ZeuzemI Schulz
Oct 1, 1992·Proceedings of the National Academy of Sciences of the United States of America·S C TsaiM Vaughan
Mar 1, 1992·Proceedings of the National Academy of Sciences of the United States of America·P J PadfieldJ D Jamieson
Oct 1, 1993·Proceedings of the National Academy of Sciences of the United States of America·J ClarkR A Kahn
Dec 1, 1993·Proceedings of the National Academy of Sciences of the United States of America·L OrciJ E Rothman
Mar 15, 1993·Proceedings of the National Academy of Sciences of the United States of America·H FuR J Collier
Aug 16, 1994·Proceedings of the National Academy of Sciences of the United States of America·L A HuberM Peter
Apr 12, 1994·Proceedings of the National Academy of Sciences of the United States of America·S C TsaiM Vaughan
Jan 17, 1995·Proceedings of the National Academy of Sciences of the United States of America·S K SappersteinM G Waters
Sep 17, 1996·Proceedings of the National Academy of Sciences of the United States of America·P P PoonG C Johnston
Jan 1, 1994·Journal of Medical and Veterinary Mycology : Bi-monthly Publication of the International Society for Human and Animal Mycology·B MarescaP R Williamson
Aug 1, 1991·The Journal of Cell Biology·T OkaA Nakano
Jan 1, 1992·The Journal of Cell Biology·A L BomanK L Wilson

❮ Previous
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