Adr1 and Cat8 mediate coactivator recruitment and chromatin remodeling at glucose-regulated genes

PloS One
Rhiannon BiddickElton T Young

Abstract

Adr1 and Cat8 co-regulate numerous glucose-repressed genes in S. cerevisiae, presenting a unique opportunity to explore their individual roles in coactivator recruitment, chromatin remodeling, and transcription. We determined the individual contributions of Cat8 and Adr1 on the expression of a cohort of glucose-repressed genes and found three broad categories: genes that need both activators for full derepression, genes that rely mostly on Cat8 and genes that require only Adr1. Through combined expression and recruitment data, along with analysis of chromatin remodeling at two of these genes, ADH2 and FBP1, we clarified how these activators achieve this wide range of co-regulation. We find that Adr1 and Cat8 are not intrinsically different in their abilities to recruit coactivators but rather, promoter context appears to dictate which activator is responsible for recruitment to specific genes. These promoter-specific contributions are also apparent in the chromatin remodeling that accompanies derepression: ADH2 requires both Adr1 and Cat8, whereas, at FBP1, significant remodeling occurs with Cat8 alone. Although over-expression of Adr1 can compensate for loss of Cat8 at many genes in terms of both activation and chromatin remod...Continue Reading

References

Jan 1, 1985·Molecular & General Genetics : MGG·H Tanaka, M Tsuboi
Jul 1, 1996·Nucleic Acids Research·U GüldenerJ H Hegemann
Sep 19, 1998·Molecular and Cellular Biology·P B KomarnitskyC L Denis
Oct 6, 1998·Annual Review of Biochemistry·D G HardieM Carlson
Dec 9, 1998·Cell·F C HolstegeR A Young
Dec 19, 2001·The Journal of Biological Chemistry·Ernesto Di MauroMicaela Caserta
Nov 26, 2002·Molecular and Cellular Biology·Marilyn G Pray-GrantPatrick A Grant
Apr 5, 2003·The Journal of Biological Chemistry·Elton T YoungTrey Ideker
Jun 16, 2004·Current Opinion in Genetics & Development·Yannick Doyon, Jacques Côté
Jun 18, 2005·Science·Guo-Cheng YuanOliver J Rando
Aug 27, 2005·PLoS Biology·Chih Long LiuOliver J Rando
Nov 3, 2005·The Journal of Biological Chemistry·Yuichiro Takagi, Roger D Kornberg
Jan 24, 2006·Nature Structural & Molecular Biology·Xiaochun FanKevin Struhl
Dec 13, 2006·Molecular Cell·Eric J SteinmetzDavid A Brow
Nov 3, 2007·The Journal of Biological Chemistry·Christine TachibanaElton T Young

❮ Previous
Next ❯

Citations

Apr 29, 2009·Genetics·Sooraj RatnakumarElton T Young
Feb 9, 2010·The Journal of Biological Chemistry·Sooraj Ratnakumar, Elton T Young
Mar 19, 2011·Analytical and Bioanalytical Chemistry·Elizabeth M HumstonRobert E Synovec
Feb 7, 2012·Biochimica Et Biophysica Acta·Georgia AbateMicaela Caserta
Mar 31, 2009·Yeast·Rhiannon Biddick, Elton T Young
Feb 23, 2011·Molecular Microbiology·Juan J InfanteElton T Young
Jul 25, 2015·FEMS Yeast Research·Ömur Kayikci, Jens Nielsen
Jun 11, 2011·FEMS Microbiology Reviews·Claudio De Virgilio
Dec 17, 2015·Molecular and Cellular Biology·Katherine A BraunElton T Young
Dec 22, 2009·Molecular BioSystems·Arthur Wuster, M Madan Babu
May 26, 2010·The Journal of Biological Chemistry·Gianpiero SpedaleW W M Pim Pijnappel
Nov 10, 2013·Bioscience, Biotechnology, and Biochemistry·Daisuke WatanabeHitoshi Shimoi
Oct 31, 2014·The Journal of Biological Chemistry·Pabitra K ParuaElton T Young
Jul 12, 2020·Applied Microbiology and Biotechnology·Özge Kalender, Pınar Çalık
Dec 28, 2016·Nucleic Acids Research·Eva KlopfChristoph Schüller
Oct 27, 2017·FEMS Microbiology Reviews·Jürgen J Heinisch, Rosaura Rodicio

❮ Previous
Next ❯

Methods Mentioned

BETA
PCR
immunoprecipitation
ChIP
histone
histone acetylation
nucleosome scanning
acetylation
immunoprecipitations

Software Mentioned

SAGA
Mediator
NuSA

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.