PMID: 8597483Dec 29, 1995Paper

Adrenal androgen biosynthesis with special attention to P450c17

Annals of the New York Academy of Sciences
J I MasonW E Rainey

Abstract

In vitro studies of human adrenal androgen synthesis are limited because of the difficulties in obtaining adrenals. We describe the use of the human adrenocortical tumor H295 cell line as a model to evaluate mechanisms controlling C19-steroid production. The cells were characterized with regard to responsiveness to a variety of agents as measured by steroid secretion and induction of 17 alpha-hydroxylase cytochrome P450 (P450c17) expression, a key enzyme in C19-steroid production. Forskolin and dibutyryl cAMP, which were more effective than ACTH, enhanced the production of DHEA and androstenedione over a 48-hour treatment period. Agents that act by increasing intracellular calcium (angiotensin II and K+ ions) as well as protein kinase A pathway activators (ACTH, forskolin, and dibutyryl cAMP) individually increased the mRNA levels and activity of P450c17. In addition, angiotensin II but not K+ ions attenuated the increased expression promoted by the kinase A agonists. Thus, the complexity of human adrenal P450c17 expression through multiple signaling pathways may contribute importantly to the diverse patterns of human adrenocortical steroidogenesis.

References

Jan 1, 1991·The Journal of Steroid Biochemistry and Molecular Biology·C C Moore, W L Miller
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Dec 1, 1992·The Journal of Steroid Biochemistry and Molecular Biology·I Hanukoglu

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Citations

Mar 11, 2008·The Journal of Steroid Biochemistry and Molecular Biology·Andres Gottfried-BlackmoreKaren Bulloch
Feb 2, 1999·Endocrinology·I H Zwain, S S Yen
Dec 20, 2000·American Journal of Physiology. Endocrinology and Metabolism·F Van CoppenolleN Prevarskaya

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