PMID: 2357528Apr 23, 1990

Adrenal steroid type I and type II receptor binding: estimates of in vivo receptor number, occupancy, and activation with varying level of steroid

Brain Research
R L SpencerB S McEwen


Adrenal steroid (AS) receptors differ from other steroid receptors in the inability of the activated form of the cytosolic receptor to exchange ligand in an in vitro binding assay. We extended this finding by demonstrating that AS receptors extracted from isolated brain nuclei also failed to exchange ligand. Taking advantage of this unique feature of AS receptors, we measured type I and type II AS binding level in rats with varying amounts of endogenous glucocorticoids or exogenous dexamethasone (DEX). We estimated the degree of receptor occupation/activation in various brain areas and the pituitary during basal glucocorticoid conditions and after acute stress. There was a variable proportion of type I receptors in the hippocampus which were unactivated during basal conditions (0-35%). The proportion of unactivated type I receptors increased (55-65%) after DEX treatment. The hippocampus was especially sensitive to the ability of low basal corticosterone (CORT) levels to activate both type I and type II receptors, whereas the pituitary was very insensitive, evidenced by a failure of acute stress levels of endogenous glucocorticoids to occupy/activate type II receptors in the pituitary. Comparison of estimates of the degree of in...Continue Reading


Mar 4, 2005·Hippocampus·Kristen L BrunsonRoland A Bender
May 22, 2001·Journal of Medical Virology·O SaridM Margalith
Aug 1, 1993·Cellular and Molecular Neurobiology·J P Herman
Nov 1, 1995·Brain Research. Molecular Brain Research·P PompeiB S McEwen
Jul 1, 1994·Molecular and Cellular Endocrinology·V K PatchevG P Chrousos
Jan 1, 1995·Psychoneuroendocrinology·A J Roberts, L D Keith
Dec 3, 2003·Trends in Endocrinology and Metabolism : TEM·Elise P Gomez-Sanchez, Celso E Gomez-Sanchez
Oct 18, 2000·European Journal of Pharmacology·J M ReulA C Linthorst
Mar 26, 2002·Psychoneuroendocrinology·Paul A CotterStuart A Checkley
Jun 10, 1998·Psychoneuroendocrinology·J W NewcomerG Vogler
Dec 17, 1998·Psychoneuroendocrinology·D M Vázquez
Apr 25, 2000·The Journal of Steroid Biochemistry and Molecular Biology·P D PatelA F Schatzberg


Aug 15, 1978·European Journal of Pharmacology·N W PedigoH I Yamamura
May 1, 1977·Molecular and Cellular Endocrinology·E R De KloetG H Mulder
Oct 1, 1989·Neuroendocrinology·H M ChaoB S McEwen
Jan 1, 1987·Molecular Endocrinology·J N VanderbiltK R Yamamoto
Jan 1, 1985·Endocrine Reviews·M R Walters
Oct 1, 1987·Endocrinology·W Sutanto, E R De Kloet
Jun 1, 1982·Endocrinology·H D VeldhuisE R De Kloet
Jan 1, 1980·Journal of Steroid Biochemistry·M Moguilewsky, J P Raynaud

Related Concepts

Corticosterone Assay
Glucocorticoid inhalants for obstructive airway disease
August Rats
Pituitary Diseases
Cytosolic receptor
Glucocorticoids, Systemic
Receptors, Steroid
Genus Hippocampus
Structure of Hippocampal Formation

Trending Feeds


Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Synapse Loss as Therapeutic Target in MS

As we age, the number of synapses present in the human brain starts to decline, but in neurodegenerative diseases this occurs at an accelerated rate. In MS, it has been shown that there is a reduction in synaptic density, which presents a potential target for treatment. Here is the latest research on synapse loss as a therapeutic target in MS.

Artificial Intelligence in Cardiac Imaging

Artificial intelligence (ai) techniques are increasingly applied to cardiovascular (cv) medicine in cardiac imaging analysis. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

Social Learning

Social learning involves learning new behaviors through observation, imitation and modeling. Follow this feed to stay up to date on the latest research.

Cell Atlas of the Human Eye

Constructing a cell atlas of the human eye will require transcriptomic and histologic analysis over the lifespan. This understanding will aid in the study of development and disease. Find the latest research pertaining to the Cell Atlas of the Human Eye here.

Single Cell Chromatin Profiling

Techniques like ATAC-seq and CUT&Tag have the potential to allow single cell profiling of chromatin accessibility, histones, and TFs. This will provide novel insight into cellular heterogeneity and cell states. Discover the latest research on single cell chromatin profiling here.

Genetic Screens in iPSC-derived Brain Cells

Genetic screening is a critical tool that can be employed to define and understand gene function and interaction. This feed focuses on genetic screens conducted using induced pluripotent stem cell (iPSC)-derived brain cells.