PMID: 2357528Apr 23, 1990

Adrenal steroid type I and type II receptor binding: estimates of in vivo receptor number, occupancy, and activation with varying level of steroid

Brain Research
R L SpencerB S McEwen

Abstract

Adrenal steroid (AS) receptors differ from other steroid receptors in the inability of the activated form of the cytosolic receptor to exchange ligand in an in vitro binding assay. We extended this finding by demonstrating that AS receptors extracted from isolated brain nuclei also failed to exchange ligand. Taking advantage of this unique feature of AS receptors, we measured type I and type II AS binding level in rats with varying amounts of endogenous glucocorticoids or exogenous dexamethasone (DEX). We estimated the degree of receptor occupation/activation in various brain areas and the pituitary during basal glucocorticoid conditions and after acute stress. There was a variable proportion of type I receptors in the hippocampus which were unactivated during basal conditions (0-35%). The proportion of unactivated type I receptors increased (55-65%) after DEX treatment. The hippocampus was especially sensitive to the ability of low basal corticosterone (CORT) levels to activate both type I and type II receptors, whereas the pituitary was very insensitive, evidenced by a failure of acute stress levels of endogenous glucocorticoids to occupy/activate type II receptors in the pituitary. Comparison of estimates of the degree of in...Continue Reading

References

Mar 4, 2005·Hippocampus·Kristen L BrunsonRoland A Bender
May 22, 2001·Journal of Medical Virology·O SaridM Margalith
Aug 1, 1993·Cellular and Molecular Neurobiology·J P Herman
Nov 1, 1995·Brain Research. Molecular Brain Research·P PompeiB S McEwen
Jul 1, 1994·Molecular and Cellular Endocrinology·V K PatchevG P Chrousos
Jan 1, 1995·Psychoneuroendocrinology·A J Roberts, L D Keith
Dec 3, 2003·Trends in Endocrinology and Metabolism : TEM·Elise P Gomez-Sanchez, Celso E Gomez-Sanchez
Oct 18, 2000·European Journal of Pharmacology·J M ReulA C Linthorst
Mar 26, 2002·Psychoneuroendocrinology·Paul A CotterStuart A Checkley
Jun 10, 1998·Psychoneuroendocrinology·J W NewcomerG Vogler
Dec 17, 1998·Psychoneuroendocrinology·D M Vázquez
Apr 25, 2000·The Journal of Steroid Biochemistry and Molecular Biology·P D PatelA F Schatzberg

Citations

Aug 15, 1978·European Journal of Pharmacology·N W PedigoH I Yamamura
May 1, 1977·Molecular and Cellular Endocrinology·E R De KloetG H Mulder
Oct 1, 1989·Neuroendocrinology·H M ChaoB S McEwen
Jan 1, 1987·Molecular Endocrinology·J N VanderbiltK R Yamamoto
Jan 1, 1985·Endocrine Reviews·M R Walters
Oct 1, 1987·Endocrinology·W Sutanto, E R De Kloet
Jun 1, 1982·Endocrinology·H D VeldhuisE R De Kloet
Jan 1, 1980·Journal of Steroid Biochemistry·M Moguilewsky, J P Raynaud

Related Concepts

Corticosterone Assay
Glucocorticoid inhalants for obstructive airway disease
August Rats
Pituitary Diseases
Brain
Cytosolic receptor
Glucocorticoids, Systemic
Receptors, Steroid
Genus Hippocampus
Structure of Hippocampal Formation

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