PMID: 43140Oct 15, 1979

Adrenaline and the regulation of acetyl-coenzyme A carboxylase in rat epididymal adipose tissue. Inactivation of the enzyme is associated with phosphorylation and can be reversed on dephosphorylation

The Biochemical Journal
R W BrownseyR M Denton

Abstract

1. Exposure of rat epididymal fat-pads or isolated fat-cells to adrenaline results in a decrease in acetyl-CoA carboxylase activity measured both in initial extracts and in extracts incubated with potassium citrate; in addition the concentration of citrate required to give half-maximal activation may also be increased. 2. Incorporation of 32Pi into acetyl-CoA carboxylase within intact fat-cells was investigated and evidence is presented that adrenaline increases the extent of phosphorylation of the enzyme. 3. Dephosphorylation of 32P-labelled acetyl-CoA carboxylase was studied in cell extracts. The rate of release of 32P is increased by 5mM-MgCl2 plus 10--100 microM-Ca2+, whereas it is inhibited by the presence of bivalent metal ion chelators such as EDTA and citrate. 4. The effects of adrenaline on the kinetic properties of acetyl-CoA carboxylase disappear if pad or cell extracts are treated with Mg2+ and Ca2+ under conditions that also lead to dephosphorylation of the enzyme. 5. The results of this study represent convincing evidence that adrenaline inactivates acetyl-CoA carboxylase in adipose-tissue preparations by increasing the degree of phosphorylation of the enzyme.

Citations

Feb 1, 1982·CRC Critical Reviews in Biochemistry·D A Flockhart, J D Corbin
Mar 8, 2005·Biochimica Et Biophysica Acta·Michael C BarberMaureen T Travers
Aug 15, 1984·Analytical Biochemistry·S R Davio, P A Fontelo
Mar 15, 1989·Biochemical and Biophysical Research Communications·M C Olivieri, L H Botelho
Dec 1, 1983·Archives of Biochemistry and Biophysics·J P TipperL A Witters
Sep 1, 1983·Archives of Biochemistry and Biophysics·B A Lent, K H Kim
Dec 16, 1983·Biochemical and Biophysical Research Communications·S Ramakrishna, W B Benjamin
Feb 7, 2001·The Journal of Biological Chemistry·A N BooneR W Brownsey
Aug 25, 1980·FEBS Letters·D H Williamson
Oct 27, 2005·The Journal of Biological Chemistry·Weissy M LeeR W Brownsey
Jul 5, 1983·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·R M Denton, R W Brownsey

Related Concepts

Acetyl-CoA Carboxylase
Fat Pad
Calcium
Citrates
Densitometry
Epididymis
Medihaler-Epi
Ligase
Magnesium
Orthophosphate

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