Adrenergic regulation of salt and fluid secretion in human medullary collecting duct cells

American Journal of Physiology. Renal Physiology
Darren P WallacePaul Pietrow

Abstract

Transepithelial salt and fluid secretion mediated by cAMP in initial inner medullary collecting ducts (IMCDi) may be important for making final adjustments to urine composition. We examined in primary cultures of human IMCDi cells the effects of adrenergic receptor (AR) agonists and antagonists on intracellular cAMP levels, short-circuit current (I(SC)), and fluid secretion. Epinephrine (1 microM), norepinephrine (1 microM), and isoproterenol (10 nM) individually increased intracellular cAMP levels 57-, 2-, and 25-fold, respectively, and stimulated I(SC) 3.3-, 2.9-, and 3.4-fold, respectively. beta-AR activation increased net fluid secretion by cultured human IMCDi cell monolayers from 0.09 +/- 0.04 to 0.26 +/- 0.05 microl x h(-1) x cm(-2) and freshly isolated rat IMCDi from 0.02 +/- 0.01 to 0.09 +/- 0.02 nl x h(-1) x mm(-1). In monolayers, these effects were eliminated by blocking beta2-AR, but not beta1-AR. Activation of alpha2-AR with guanabenz inhibited isoproterenol-induced I(SC) by 37% in human IMCDi monolayers and fluid secretion by 91% in rat IMCDi. Immunohistochemistry of human medullary tissue sections revealed greater expression of beta2-AR than beta1-AR; beta2-AR was localized to the basolateral membranes of human I...Continue Reading

References

Feb 1, 1975·The American Journal of Physiology·H Sonnenberg
Aug 1, 1992·The American Journal of Physiology·Y MaedaM A Knepper
Nov 1, 1991·The American Journal of Physiology·G YasudaW B Jeffries
Oct 1, 1991·Canadian Journal of Physiology and Pharmacology·B A Stanton
Jun 1, 1991·The American Journal of Physiology·R Keeler, A M Azzarolo
May 1, 1990·The American Journal of Physiology·C S Wingo
Jan 1, 1990·The American Journal of Physiology·S M WallM A Knepper
Jan 1, 1985·Journal of Cardiovascular Pharmacology·W Kriz, S Bachmann
Sep 1, 1988·Proceedings of the National Academy of Sciences of the United States of America·J W ReganB K Kobilka
Jul 1, 1985·Proceedings of the National Academy of Sciences of the United States of America·S M ShreeveJ C Venter
Jun 1, 1985·The American Journal of Physiology·D D SmythW A Pettinger
Jul 1, 1985·The American Journal of Physiology·S UmemuraW A Pettinger
Jan 1, 1973·The American Journal of Physiology·R G Luke
Jan 1, 1973·The American Journal of Physiology·R G Luke
Sep 25, 1984·Biochemistry·J L BenovicR J Lefkowitz
Jul 1, 1984·The American Journal of Physiology·L BarajasP Wang
Sep 1, 1995·The American Journal of Physiology·D VandorpeB A Stanton
May 1, 1995·The American Journal of Physiology·N L KizerB A Stanton
Aug 1, 1993·The American Journal of Physiology·M L Zeidel
Jul 1, 1996·The American Journal of Physiology·A J Rouch, L H Kudo
Sep 1, 1996·The American Journal of Physiology·G YasudaW B Jeffries
Oct 1, 1996·Kidney International·D P WallaceL P Sullivan
Dec 1, 1996·Journal of the American Society of Nephrology : JASN·S M GinnsJ B Wade
Jan 1, 1997·Kidney International·M G ZieglerB Kennedy
Jan 1, 1997·Physiological Reviews·G F DiBona, U C Kopp
Feb 1, 1997·The American Journal of Physiology·G YasudaW B Jeffries
Mar 1, 1997·The American Journal of Physiology·D H VandorpeB A Stanton
Nov 15, 1997·The American Journal of Physiology·S L AlperD Brown
Jun 5, 1998·The American Journal of Physiology·J B Stokes, R D Sigmund
Jun 11, 1998·Journal of the American Society of Nephrology : JASN·A K Stuart-TilleyS L Alper
Aug 5, 1998·The American Journal of Physiology·T W WilbornJ A Schafer
Oct 12, 2000·Anais Da Academia Brasileira De Ciências·M M MoralesA G Lopes
Dec 16, 2000·Clinical and Experimental Pharmacology & Physiology·S C Malpas, B L Leonard

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Citations

Dec 4, 2009·American Journal of Physiology. Renal Physiology·Kevin A StraitDonald E Kohan
Dec 25, 2012·Journal of the American Society of Nephrology : JASN·Karl P RoosDonald E Kohan
Aug 21, 2015·The Journal of Membrane Biology·Kyle JanssonGustavo Blanco
Apr 28, 2017·Hypertension Research : Official Journal of the Japanese Society of Hypertension·Yusaku MoriTsutomu Hirano
Mar 17, 2016·World Journal of Biological Chemistry·Luciana MorlaGilles Crambert
May 16, 2009·Clinical Journal of the American Society of Nephrology : CJASN·Vicente E TorresJared J Grantham
May 31, 2014·Clinical Journal of the American Society of Nephrology : CJASN·David PearceDonald E Kohan
Jan 13, 2015·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Weiling WangBaoxue Yang
Jul 1, 2015·Current Opinion in Nephrology and Hypertension·Madhumitha Rajagopal, Darren P Wallace

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