Adrenergic stimulation alters the expression of inflammasome components and interleukins in primary human monocytes

Experimental and Therapeutic Medicine
J P HorstmannBorna Relja

Abstract

Prior to their release, interleukin (IL)-1β and IL-18 are cleaved to their bioactive forms by a multiprotein complex known as an inflammasome, which is comprised of a number of elements that are subject to nuclear factor-κB-dependent transcription. Catecholamines have been indicated to exert an enhancing effect on the IL-1β release. The aim of the present study was to determine whether alterations in inflammasome gene expression may be responsible for the modified IL-1β and IL-18 secretion following lipopolysaccharide (LPS) and catecholamine co-stimulation. Monocytes were isolated from the peripheral blood of 21 healthy volunteers using CD14(+) microbeads. Following stimulation with LPS (2 µg/ml) and/or phenylephrine (PE; 10 µM) for 24 h, the supernatants were subjected to ELISA to evaluate the ex vivo protein expression levels of IL-1β and IL-18. In addition, the gene expression levels of inflammasome components associated with the cleavage of IL-1β and IL-18, including NLRP1, NLRP3, caspase-1 and PYCARD were determined using polymerase chain reaction. The results indicated that LPS significantly increased IL-1β expression compared with the unstimulated control samples. Co-stimulation with LPS + PE significantly enhanced IL-1β...Continue Reading

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Citations

Aug 14, 2019·Microbiology and Immunology·Gunlög RasmussenBo Söderquist
Jan 9, 2019·Molecular Medicine·Rose A WillemzeWouter J de Jonge
Jul 24, 2020·Clinical Autonomic Research : Official Journal of the Clinical Autonomic Research Society·David S Goldstein
Jan 1, 2021·BioMed Research International·Grzegorz NiemczykAgnieszka Cudnoch-Jedrzejewska
Feb 26, 2020·Pharmacology & Therapeutics·Anita A PinarChrishan S Samuel

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