PMID: 8969922Nov 1, 1996Paper

Adrenocortical-specific transgene expression directed by steroid hydroxylase gene promoters

Endocrine Research
S D MorleyJ J Mullins

Abstract

The 5'-flanking regions of genes for three mouse adrenal steroid hydroxylases were analyzed for their ability to direct adrenal cortex-specific beta-galactosidase (beta-gal) reporter expression both in cell culture and transgenic mice. The 5'-flanking regions chosen were from the genes for steroid 21-hydroxylase (21-OHase), expressed throughout the adrenal cortex and mediating both glucocorticoid and mineralocorticoid synthesis, and aldosterone synthetase (AS) and steroid 11 beta-hydroxylase (11 beta-OHase), which catalyze respectively the terminal steps of mineralocorticoid synthesis in the zona glomerulosa and glucocorticoid synthesis in the zona fasciculata/reticularis. While 5.0 kb of 11 beta-OHase gene 5'-flanking region and 5.4 kb of the AS gene 5'-flanking region mediated respectively moderate and low levels of beta-gal reporter expression in Y1 adrenocortical tumor cells, neither of these 5'-flanking regions was able to direct reporter expression to the appropriate adrenocortical zone of transgenic mice. This suggests that additional regulatory elements, lying outside these 5'-flanking regions, are required for 11 beta-OHase and AS gene expression in the intact mouse. In contrast, 6.4 kb of the mouse 21-OHase A gene 5' ...Continue Reading

References

Oct 1, 1991·Molecular Endocrinology·D A RiceK L Parker
May 1, 1990·Molecular and Cellular Biology·K ZimmermanF W Alt
Jan 1, 1989·Endocrine Research·J D HandlerK L Parker
Jan 1, 1985·Methods in Enzymology·B P Schimmer
Jun 11, 1987·The New England Journal of Medicine·P C WhiteB Dupont

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Citations

Dec 24, 2010·Anatomia, histologia, embryologia·S M El-NahlaL C Abbott
Dec 12, 2002·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Philip IannacconeGabriel Landini
Jan 7, 1999·Brazilian Journal of Medical and Biological Research = Revista Brasileira De Pesquisas Médicas E Biológicas·M Bader
Aug 20, 2003·European Journal of Clinical Investigation·Y WangG Vassaux
Feb 28, 2013·Journal of Cell Science·Marcin ChruscielNafis A Rahman

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