Adrenomedullin 2 protects rat cerebral endothelial cells from oxidative damage in vitro

Brain Research
Lei ChenYoichi Ueta

Abstract

Adrenomedullin 2 (AM2, intermedin) is a recently identified new member of the calcitonin gene-related peptide family. We examined whether AM2 can attenuate the increased blood-brain barrier permeability and cerebral endothelial cell (CEC) death induced by oxidative stress in vitro. Hydrogen peroxide (H(2)O(2), 0.5 mM) induced a continuous decrease of the transendothelial electrical resistance (TEER) and resulted in intercellular gap formations in rat CECs co-cultured with astrocytes. AM2 induced cAMP and nitric oxide production, increased TEER, enhanced peripheral localization of F-actin bands, and attenuated the increased permeability induced by H(2)O(2). AM2 treatment preserved mitochondrial membrane potential and improved CEC viability in H(2)O(2) treated cultures. These effects of AM2 were similar to those what were reported for adrenomedullin. These results suggest that AM2 protects CECs against oxidative injury in vitro.

References

Dec 1, 1991·The Journal of Cell Biology·L L RubinJ Morales
Mar 30, 1999·European Journal of Pharmacology·B KisM A Deli
Apr 15, 1999·Annual Review of Neuroscience·L L Rubin, J M Staddon
Apr 28, 1999·Free Radical Biology & Medicine·S López-OngilD Rodríguez-Puyol
Jan 4, 2001·European Journal of Pharmacology·M A DeliM Niwa
Mar 27, 2001·Seikagaku. The Journal of Japanese Biochemical Society·K Takahashi
Jan 5, 2002·Circulation·Tatsuo ShimosawaToshiro Fujita
Apr 6, 2002·Circulation Research·Toshihiro Tsuruda, John C Burnett
Apr 20, 2002·Journal of Neuroendocrinology·B KisY Ueta
May 31, 2002·Pharmacological Reviews·Yossi Gilgun-SherkiDaniel Offen
Oct 5, 2002·Circulation Research·Stefan HippenstielNorbert Suttorp
Mar 8, 2003·Stroke; a Journal of Cerebral Circulation·J M WardlawJ Starr
Mar 13, 2003·Hypertension Research : Official Journal of the Japanese Society of Hypertension·Béla KisYoichi Ueta
Feb 11, 2004·Circulation·Junsuke KawaiToshiro Fujita
Oct 12, 2004·American Journal of Physiology. Cell Physiology·K BindewaldT Noll
May 25, 2005·Brain Research·Meghan M Taylor, Willis K Samson

❮ Previous
Next ❯

Citations

Dec 12, 2012·Cellular and Molecular Neurobiology·Shoji HoraiMasami Niwa
Jul 3, 2010·Journal of Molecular Neuroscience : MN·Kazuhiro TakahashiKazuhito Totsune
Aug 18, 2009·American Journal of Physiology. Lung Cellular and Molecular Physiology·Uwe PfeilWolfgang Kummer
Oct 3, 2008·American Journal of Physiology. Renal Physiology·Makoto HagiwaraJulie Chao
Jan 23, 2008·Cellular Physiology and Biochemistry : International Journal of Experimental Cellular Physiology, Biochemistry, and Pharmacology·David BellBarbara J McDermott
Apr 29, 2015·Clinical and Experimental Medicine·Yanhong WangRongshan Li
Jul 23, 2013·Progress in Neuro-psychopharmacology & Biological Psychiatry·Abdullah AkpinarSuleyman Onal
Sep 1, 2007·Cellular Physiology and Biochemistry : International Journal of Experimental Cellular Physiology, Biochemistry, and Pharmacology·David BellBarbara J McDermott
Dec 3, 2016·Thrombosis and Haemostasis·Dursun GündüzMuhammad Aslam
Apr 14, 2017·British Journal of Pharmacology·Song-Yang ZhangXian Wang
Mar 31, 2010·Journal of Cardiovascular Pharmacology·Jing-Hui YangYong-Fen Qi

❮ Previous
Next ❯

Related Concepts

Related Feeds

Blood Brain Barrier

The blood brain barrier is a border that separates blood from cerebrospinal fluid. Discover the latest search on this highly selective semipermeable membrane here.

Blood Brain Barrier Regulation in Health & Disease

The blood brain barrier is essential in regulating the movement of molecules and substances in and out of the brain. Disruption to the blood brain barrier and changes in permeability allow pathogens and inflammatory molecules to cross the barrier and may play a part in the pathogenesis of neurodegenerative disorders. Here is the latest research in this field.

Blood Brain Barrier Chips

The blood brain barrier (BBB) is comprised of endothelial cells that regulate the influx and outflux of plasma concentrations. Lab-on-a-chip devices allow scientists to model diseases and mechanisms such as the passage of therapeutic antibodies across the BBB. Discover the latest research on BBB chips here.

Astrocytes in Repair & Regeneration

Astrocytes are glial cells found within the CNS and are able to regenerate new neurons. They become activated during CNS injury and disease. The activation leads to the transcription of new genes and the repair and regeneration of neurons. Discover the latest research on astrocytes in repair and regeneration here.

Astrocytes

Astrocytes are glial cells that support the blood-brain barrier, facilitate neurotransmission, provide nutrients to neurons, and help repair damaged nervous tissues. Here is the latest research.