Adrenomedullin has mitogenic effects on human oral keratinocytes: involvement of cyclic AMP

FEBS Letters
S KapasE Hagi-Pavli

Abstract

The effects of the novel vasoactive regulatory peptide, adrenomedullin, on human oral keratinocytes was investigated. Adrenomedullin, acting via its specific receptor, stimulated a dose-dependent increase in DNA synthesis, and, in addition, stimulated further changes in the cell cycle resulting in the proliferation of keratinocytes. When cells were incubated in the presence of increasing concentrations of adrenomedullin, there was a rapid and dose-dependent rise in intracellular cyclic AMP levels. Stimulation of mitogenesis and cell proliferation in these cells were mimicked by the cell permeable cAMP analogue, dibutyryl cAMP. Adrenomedullin-stimulated mitogenesis was attenuated by the adenylyl cyclase inhibitor SQ22,536, but was unaffected by inhibitors of PKC, tyrosine kinase or the CGRP receptor antagonist, CGRP(8-37). These data identify adrenomedullin as a new mitogenic regulatory peptide of keratinocytes acting via the cAMP cascade.

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Citations

Jun 2, 2005·European Journal of Medicinal Chemistry·Miguel JuliánFrank Cuttitta
Nov 11, 1999·European Journal of Pharmacology·T HorioK Kangawa
Apr 2, 2003·Regulatory Peptides·Reiko Udono-FujimoriKazuhiro Takahashi
Apr 2, 2003·Regulatory Peptides·Robert P Allaker, Supriya Kapas
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Jan 22, 2003·The British Journal of Dermatology·F B MüllerM P Philpott
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Oct 11, 2012·Stem Cells International·Sonia Martínez-HerreroAlfredo Martínez
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