Abstract
Produced by vascular walls, adrenomedullin (AM) exerts antifibrotic actions in the process of cardiovascular remodeling. The purpose of this study was to examine the pathophysiological role of AM in the development of human abdominal aortic aneurysm (AAA). Immunohistochemical analyses revealed that vascular smooth muscle cells in the media were positive for AM in the early stage of atherosclerotic aorta. Intense immunoreactivity was observed in mast cells of the outer media and adventitia of AAA, and the number of mast cells was greater (p < 0.01) in AAA than in atherosclerotic aorta without any aneurysmal change. To determine the role of AM in mast cells, we examined cultured human mast cell leukemia line-1 (HMC-1) and fibroblasts isolated from AAA patients. Cultured HMC-1 cells were found to express preproAM gene and release AM peptide into the cultured media. When assessed by collagenase-sensitive [3H]proline incorporation and procollagen type I C-peptide secretion, collagen synthesis in co-culture of HMC-1 and the fibroblasts was reduced by 10(-6) mol/L synthetic AM, while conversely, it increased following blockade of the action of endogenous AM with 10 microg/mL anti-AM monoclonal antibody. The present study suggests an a...Continue Reading
Citations
Nov 3, 2010·Cardiovascular Research·Jean-Baptiste MichelUNKNOWN FAD EU consortium
Oct 30, 2007·Clinica Chimica Acta; International Journal of Clinical Chemistry·Keiichi FurubayashiHitoshi Fukumoto
Jul 2, 2009·Autonomic & Autacoid Pharmacology·S K WalshC L Wainwright
May 15, 2007·Immunological Reviews·Martin MetzStephen J Galli
Dec 28, 2010·Biochimica Et Biophysica Acta·Jie Zhang, Guo-Ping Shi
May 12, 2015·European Journal of Pharmacology·Eva KritikouIlze Bot
Jan 22, 2013·Pharmacology & Therapeutics·Simon KennedyPasquale Maffia
Jun 2, 2007·Pharmacological Reviews·Domenico RibattiGastone G Nussdorfer
Apr 10, 2010·Circulation Journal : Official Journal of the Japanese Circulation Society·Toshihiro TsurudaKazuo Kitamura
Feb 13, 2018·Immunological Reviews·Peter Bradding, Gunnar Pejler
Aug 3, 2017·Angiology·Stefan AcostaJ Gustav Smith
Mar 30, 2012·International Journal of Surgical Pathology·Maryam Sotoudeh AnvariHamidreza Goodarzynejad
Mar 25, 2020·Revista Portuguesa De Cardiologia : Orgão Oficial Da Sociedade Portuguesa De Cardiologia = Portuguese Journal of Cardiology : an Official Journal of the Portuguese Society of Cardiology·Guilherme Ribeiro JulianoMara Lúcia da Fonseca Ferraz