Adrenomedullin stimulates DNA synthesis of rat adrenal zona glomerulosa cells through activation of the mitogen-activated protein kinase-dependent cascade

Journal of Hypertension
A SempliciniG G Nussdorfer

Abstract

Adrenal zona glomerulosa cells are provided with adrenomedullin receptors. Adrenomedullin has recently been found to enhance proliferation of cultured rat vascular smooth muscle cells and zona glomerulosa cells. To investigate whether adrenomedullin affects rat zona glomerulosa proliferative activity through the tyrosine kinase and extracellular signal regulated kinases (ERKs) pathways. Dispersed rat zona glomerulosa cells were cultured in vitro for 24 h and then exposed to adrenomedullin (10(-7) mol/l), alone or in the presence of tyrphostin-23 (10(-5) mol/l) or PD-98059 (10(-4) mol/l), for 24 or 48 h. To assess the rate of DNA synthesis, 5-bromo-2'-deoxyuridine (BrdU, 20 mg/ml) was also added to the medium and BrdU-positive cells were detected by immunocytochemistry. The expression of ERKs and the effect of adrenomedullin on ERKs phosphorylation and activity were assayed in dispersed zona glomerulosa cells. Adrenomedullin significantly increased the percentage of BrdU-positive (phase-S) zona glomerulosa cells; this effect was blocked by either the tyrosine kinase inhibitor, tyrphostin-23, or the mitogen-activated protein kinase kinase (MEK-1) inhibitor, PD-98059. Both zona glomerulosa and zona fasciculata/reticularis express ...Continue Reading

References

Nov 10, 1995·Archives of Biochemistry and Biophysics·L RamdasR J Budde
Oct 24, 1995·Biochemical and Biophysical Research Communications·E N ChiniT P Dousa
Apr 1, 1999·Annual Review of Physiology·W K Samson
Nov 16, 1999·Biochimica Et Biophysica Acta·M SartoriA Semplicini
Feb 5, 2000·Nature·A J Whitmarsh, R J Davis
Sep 8, 2000·Journal of Cardiovascular Pharmacology·G P RossiA C Pessina
Sep 22, 2000·The Biochemical Journal·S P DaviesP Cohen

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Citations

Jun 2, 2005·European Journal of Medicinal Chemistry·Miguel JuliánFrank Cuttitta
Jun 8, 2002·Biochemical and Biophysical Research Communications·Carine CueilleJean-Michel Garel
Apr 2, 2003·Regulatory Peptides·Ludwik K MalendowiczGastone G Nussdorfer
Apr 2, 2003·Regulatory Peptides·Masayoshi ShichiriYukio Hirata
Jul 18, 2002·Annals of the New York Academy of Sciences·Lawrence S Kirschner
Mar 13, 2003·Hypertension Research : Official Journal of the Japanese Society of Hypertension·Gian Paolo RossiGastone G Nussdorfer
Mar 13, 2003·Hypertension Research : Official Journal of the Japanese Society of Hypertension·Masayoshi Shichiri, Yukio Hirata
Sep 1, 2004·Biochemical and Biophysical Research Communications·Ibane AbasoloAlfonso Calvo
Mar 29, 2002·Microscopy Research and Technique·Frank CuttittaAlfredo Martínez
Jun 14, 2003·Arteriosclerosis, Thrombosis, and Vascular Biology·Won KimSung Kwang Park
Mar 24, 2004·Integrative Cancer Therapies·C Murray Ardies
Apr 20, 2006·The Journal of Immunology : Official Journal of the American Association of Immunologists·Benjamin UzanFrédéric Lioté

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