May 2, 2020

Unannotated small RNA clusters in circulating extracellular vesicles detect early stage liver cancer

BioRxiv : the Preprint Server for Biology
J. v. FeldenA. Villanueva

Abstract

Liver cancer is among the deadliest malignancies and surveillance tools for early detection are suboptimal. Extracellular vesicles (EVs) have gained increasing scientific interest due to their involvement in tumor initiation and metastasis, however, most extracellular RNA (exRNA) biomarker studies are limited to annotated genomic regions. We present a novel data-driven approach that naturally includes unannotated genomic regions and facilitates comprehensive surveys of the entire small exRNA payload. We identify novel small exRNA signatures from unannotated loci, termed small RNA clusters (smRCs), and further characterize their properties across different EV isolation techniques, biofluids, and cancer types. An exRNA-based 3-smRC signature from plasma detects patients with early stage liver cancer from control patients at high risk, which directly leads to the prospect of a minimally-invasive, blood-only, operator-independent approach targeting unannotated small exRNA for early cancer detection.

  • References
  • Citations

References

  • We're still populating references for this paper, please check back later.
  • References
  • Citations

Citations

  • This paper may not have been cited yet.

Mentioned in this Paper

Doxorubicin
Computer Software
Biological Markers
Derivatives
Genes
Deoxyhypusine synthase
Amino Acids, I.V. solution additive
DHFR
Nucleic Acid Sequencing
DHPS gene

Related Feeds

Antimalarial Agents

Antimalarial agents, also known as antimalarials, are designed to prevent or cure malaria. Discover the latest research on antimalarial agents here.

BioRxiv & MedRxiv Preprints

BioRxiv and MedRxiv are the preprint servers for biology and health sciences respectively, operated by Cold Spring Harbor Laboratory. Here are the latest preprint articles (which are not peer-reviewed) from BioRxiv and MedRxiv.

Antimalarial Agents (ASM)

Antimalarial agents, also known as antimalarials, are designed to prevent or cure malaria. Discover the latest research on antimalarial agents here.