Adriamycin inhibits adipogenesis through the modulation of PPARγ and restoration of adriamycin-mediated inhibition of adipogenesis by PPARγ over-expression

Toxicology Mechanisms and Methods
Sankarganesh ArunachalamPyoung-Han Hwang

Abstract

Adriamycin is an anti-cancer drug, effective against a wide range of cancers. However, its clinical application is limited by its cardiotoxicity. A number of reports suggest that adriamycin induces bodyweight loss also. The aim of this study was to investigate the effect of adriamycin on adipogenesis as bodyweight chancges can be directly correlated with adipocytes. Fat accumulation in 3T3-L1 pre-adipocytes, as a result of adipogenesis was detected using oil red O staining. We performed western immunoblot for the expression of adipocyte differentiation related genes to analyze the molecular mechanism of adriamycin-mediated inhibition of adipogenesis. Over-expression of target gene was done by using recombinant adenoviruses. Adriamycin inhibited adipogenesis in a dose-dependent manner. It was observed that adriamycin down-regulated the expression of PPARγ. Moreover, up-stream elements of PPARγ were also found to be down-regulated by adriamycin. Adriamycin might prevent bodyweight gain through inbibition of adipogenesis by the down-regulation of PPARγ and its up-stream transcriptional regulators like C/EBPβ and KLF4. To reverse the adriamycin-mediated inhibition of adipogenesis, PPARγ was over-expressed by adenoviral mediated gen...Continue Reading

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Citations

Apr 22, 2015·Trends in Pharmacological Sciences·Luc RochetteCatherine Vergely
Mar 26, 2016·PloS One·Luana Amorim BiondoJosé Cesar Rosa Neto
Oct 29, 2015·Current Opinion in Supportive and Palliative Care·Fábio S LiraJosé C Rosa Neto
Mar 28, 2020·Circulation Research·Kendall B WallacePaulo J Oliveira
Mar 31, 2018·Current Medicinal Chemistry·Robert HromadkaVladimir Kral
Oct 28, 2017·European Journal of Pharmacology·Kaviyarasi RenuSankarganesh Arunachalam
Apr 21, 2021·Reproductive Toxicology·Uma Priya MohanSankarganesh Arunachalam
Jul 17, 2019·Chemical Research in Toxicology·Syeda Thabassum Akhtar IqbalSankarganesh Arunachalam

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