ADRP is dissociated from lipid droplets by ARF1-dependent mechanism

Biochemical and Biophysical Research Communications
Noriko NakamuraToyoshi Fujimoto

Abstract

Adipocyte differentiation-related protein (ADRP) is a member of PAT proteins existing in lipid droplets (LDs). By yeast two-hybrid screening, we identified ADP-ribosylation factor 1 (ARF1) as a binding partner of ADRP. The interaction of ADRP and ARF1 was verified by GST pull-down and co-immunoprecipitation experiments. Interestingly, ADRP precipitated the GDP-bound ARF1 preferentially to the GTP-bound ARF1. Consistent with this, either brefeldin A (BFA), a fungal metabolite to inhibit ARF-GEF, or a dominant-negative mutant of ARF1 caused dissociation of ADRP from LD. On the other hand, overexpression of wild-type ARF1 did not promote the ADRP dissociation or new LD formation. By using deletion mutants, a central domain of ADRP, which is dispensable for LD binding, was shown to bind to ARF1. The present study showed that the GDP-bound ARF1 induces dissociation of ADRP from the LD surface, and that LD is a target of BFA action.

References

Sep 18, 1997·The Journal of Experimental Medicine·P T BozzaP F Weller
Jul 29, 1998·Histochemistry and Cell Biology·A Dinter, E G Berger
Apr 27, 1999·Trends in Biochemical Sciences·D J Murphy, J Vance
Jun 4, 1999·Seminars in Cell & Developmental Biology·C LondosA R Kimmel
Mar 10, 2001·The Journal of Cell Biology·T FujimotoR Nomura
May 3, 2001·The Journal of Biological Chemistry·B P AtshavesF Schroeder
May 24, 2001·Proceedings of the National Academy of Sciences of the United States of America·J T TanseyC Londos
Dec 18, 2001·The Journal of Biological Chemistry·Nelson B ColeRobert L Nussbaum
Sep 10, 2002·American Journal of Physiology. Endocrinology and Metabolism·Minako ImamuraHajime Nawata
Sep 11, 2002·The Journal of Biological Chemistry·Kumi Tauchi-SatoToyoshi Fujimoto
Oct 31, 2002·The Journal of Biological Chemistry·Anne GarciaDawn L Brasaemle
Feb 4, 2003·Journal of Lipid Research·James L McManamanDavid J Orlicky
Feb 20, 2003·The Journal of Biological Chemistry·Paul Targett-AdamsJohn McLauchlan
Jun 14, 2003·Biochemical and Biophysical Research Communications·Noriko Nakamura, Toyoshi Fujimoto
Jun 18, 2003·The Journal of Cell Biology·Carole SztalrydConstantine Londos
Jul 3, 2003·The Journal of Biological Chemistry·Masato OhashiTamotsu Yoshimori
Aug 2, 2003·Current Opinion in Cell Biology·Zhongzhen NiePaul A Randazzo
Nov 5, 2003·The Journal of Biological Chemistry·Pingsheng LiuRichard G W Anderson
Dec 9, 2003·The Journal of Biological Chemistry·Yoko ShibaKazuhisa Nakayama
Jan 8, 2004·Cellular Signalling·Paul A Randazzo, Dianne S Hirsch
Mar 17, 2004·The Journal of Biological Chemistry·Ellen UmlaufRainer Prohaska
Jul 10, 2004·Structure·Sabrina J HickenbottomJames H Hurley

❮ Previous
Next ❯

Citations

Mar 22, 2006·Nature Reviews. Molecular Cell Biology·Sally Martin, Robert G Parton
Jun 1, 2007·Molecular Biology of the Cell·René BartzPingsheng Liu
Jul 23, 2013·Plant & Cell Physiology·Naohiro KatoDrury Ingram
Mar 23, 2011·Cold Spring Harbor Perspectives in Biology·Toyoshi Fujimoto, Robert G Parton
Nov 12, 2010·Journal of Virology·Meirav MattoBenjamin Aroeti
May 25, 2013·Annual Review of Nutrition·Kelly V RugglesStephen L Sturley
Dec 8, 2009·Journal of Lipid Research·Brittany D M Hodges, Christine C Wu
May 23, 2009·Journal of Cell Science·Krishnakant G SoniJuan S Bonifacino
Dec 11, 2008·PLoS Biology·Mathias BellerBrian Oliver
May 15, 2012·Annual Review of Cell and Developmental Biology·Hector Alex Saka, Raphael Valdivia
Mar 23, 2010·FEBS Letters·Mathias BellerHerbert Jäckle
Apr 21, 2009·Biochimica Et Biophysica Acta·Perry E BickelMichael A Welte
Jan 17, 2009·Biochimica Et Biophysica Acta·Gerd Schmitz, Margot Grandl
Jan 23, 2009·Proteomics·John K ZehmerPingsheng Liu
Jul 1, 2006·Biochemical and Biophysical Research Communications·Yuki OhsakiToyoshi Fujimoto
Aug 2, 2005·Biochemical and Biophysical Research Communications·Noriko NakamuraKeiko Tamiya-Koizumi
Sep 1, 2015·Frontiers in Cell and Developmental Biology·Qiang Gao, Joel M Goodman
Aug 31, 2014·Biochemical and Biophysical Research Communications·Arthur SlettenLaura L Listenberger
May 10, 2016·Biochemical and Biophysical Research Communications·Na ZhangLeiliang Zhang
Nov 15, 2014·PloS One·Joan GannonTommy Nilsson
Oct 24, 2006·Journal of Lipid Research·Masafumi NagayamaKazutoshi Gohara
Aug 16, 2005·The Journal of Biological Chemistry·Dorothee LayWilhelm W Just
Dec 15, 2010·Molecular Membrane Biology·Fariba KalantariTommy Nilsson
Jul 13, 2017·Neurochemical Research·Xirong HuWei Ge
Jul 5, 2007·Journal of Proteome Research·René BartzPingsheng Liu
Aug 13, 2021·Journal of Cell Science·Benjamin S RobertsSaskia B Neher

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.

© 2021 Meta ULC. All rights reserved