PMID: 8957705Jan 1, 1996Paper

Adsorbed serum protein mediated adhesion and growth behavior of bovine aortic endothelial cells on polyamine graft copolymer surfaces

Journal of Biomaterials Science. Polymer Edition
A KikuchiK Kataoka

Abstract

Polyamine-brushed substrata for cell culture were designed by solvent casting of polystyrene-graft-polyamine copolymer (SA) on hydrophobically modified glass surface, and adhesion, spreading, and proliferation of bovine aortic endothelial cells (BAEC) on these substrata were evaluated. Adhesion and spreading of BAEC increased with increasing polyamine content in the copolymer. Close correlation was found between cellular spreading and subsequent cell growth; the surface inducing better spreading of adhered cells showed higher endothelial cell growth. Adhesion and spreading of BAEC were significantly influenced by fetal calf serum (FCS)-pretreatment of the SA copolymer surfaces, being increased with increasing polyamine content, whereas on bovine serum albumin (BSA)-preabsorbed surfaces, BAEC adhesion was considerably prevented and eventually no cell spreading was observed. Then, adsorption of cell-adhesion proteins, fibronectin (FN), and vitronectin (VN), out of FCS onto SA copolymer surfaces were evaluated using enzyme immunoassay. Both FN and VN adsorption on SA copolymer surfaces were increased with increasing polyamine content in the copolymer, suggesting a crucial role of these cell-adhesion proteins in BAEC adhesion and s...Continue Reading

References

Sep 1, 1975·Journal of Biomedical Materials Research·B D RatnerS D Hauschka
Jun 1, 1978·Nature·J Folkman, A Moscona
Dec 1, 1976·The Journal of Cell Biology·W L McKeehan, R G Ham
Jan 1, 1991·Journal of Biomaterials Science. Polymer Edition·D J Fabrizius-Homan, S L Cooper
Jan 1, 1991·Annual Review of Cell Biology·K T Preissner
Jan 1, 1990·Journal of Biomaterials Science. Polymer Edition·J Jozefonvicz, M Jozefowicz
Jan 1, 1987·Annals of the New York Academy of Sciences·F Grinnell
Nov 1, 1982·The Journal of Cell Biology·R O Hynes, K M Yamada
Jun 1, 1983·Analytical Biochemistry·T R Downs, W W Wilfinger
May 1, 1981·Journal of Biomedical Materials Research·F Grinnell, M K Feld
Jan 1, 1994·Journal of Biomaterials Science. Polymer Edition·H J GriesserJ G Steele

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Citations

Jan 5, 2002·Biomaterials·Sharon L SanbornKandice Kottke-Marchant
Mar 2, 2005·Tissue Engineering·Cameron J WilsonMark J Pearcy
Jan 1, 2009·Tissue Engineering. Part a·Shivali DuggalJan E Brinchmann
Sep 4, 2012·Journal of Biomedical Materials Research. Part a·Dave MangindaanMeng-Jiy Wang
Oct 17, 2012·Biomaterials·Yi HongJun Lin
Mar 19, 2013·Journal of Controlled Release : Official Journal of the Controlled Release Society·Omar QutachiLee D K Buttery
Jan 18, 2006·Journal of Biomaterials Science. Polymer Edition·F BianchiA Ahluwalia
Feb 25, 2010·Journal of Biomaterials Science. Polymer Edition·Wei-Bor TsaiJuin-Yih Lai
Mar 16, 2005·Macromolecular Bioscience·Wei-Bor Tsai, Min-Cheng Wang
Jun 18, 2002·Journal of Cellular Physiology·Mercedes Balcells, Elazer R Edelman
Jun 28, 2001·Journal of Biomedical Materials Research·G RainaldiM T Santini
Jan 19, 2021·ACS Biomaterials Science & Engineering·Xiaojun LongJun Lin
Jan 20, 2021·ACS Biomaterials Science & Engineering·Cunyang WangXiaoming Li

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