PMID: 7545003Jan 1, 1995Paper

Adsorption and covalent immobilization of human serum albumin (HSA) and gamma globulins (gamma G) onto poly(styrene/acrolein) latexes with pyrene, dansyl, and 2,4-dinitrophenyl labels

Journal of Biomaterials Science. Polymer Edition
B Miksa, S Slomkowski

Abstract

The poly(styrene/acrolein) latexes (P(SA)1 and P(SA)2), differing in poly(acrolein) content, were synthesized by the emulsifier-less emulsion-precipitation polymerization of styrene and acrolein. The fraction of poly(acrolein) in the surface layer was 0.35 and 0.50, for the P(SA)1 and P(SA)2 latex, respectively. Latexes were labelled with 2,4-dinitrophenylhydrazine (DNPH), dansylhdrazine (DAH), and 1-aminopyrene (APY). Surface concentration of labels varied from 4.20.10(-7) mol m-2 (for APY label on P(SA)1 latex) to 1.54.10(-6) mol m-2 (for DNPH label on P(SA)2 latex) reflecting the fraction of polyacrolein in the surface layer and bulkiness of the label. The differences between adsorption and covalent immobilization of human serum albumin and gamma globulins onto the P(SA)2 latex and onto its derivatives labelled with the 2,4-dinitrophenyl (DNP), dansyl (DA), and pyrene (PY) groups were small. The observation conforms to the hypothesis that polyacrolein forms domains on the surface of the P(SA) latexes and that after labelling some aldehyde groups are still available for the covalent immobilization of proteins. Labelled and parent latexes were used in the model slide and turbidimetric aggregation tests for the goat anti-HSA. T...Continue Reading

References

Oct 1, 1979·Journal of Chromatography·P D Dean, D H Watson
Jul 16, 1992·Nature·X M He, D C Carter
Jan 1, 1991·Journal of Biomaterials Science. Polymer Edition·T Basinska, S Slomkowski
Jan 1, 1991·Journal of Clinical Laboratory Analysis·L BorqueR Ruiz
Jan 1, 1985·Advances in Protein Chemistry·T Peters
May 1, 1968·American Journal of Clinical Pathology·V S BolinJ B Alsever
Jul 1, 1973·American Journal of Obstetrics and Gynecology·C A HorwitzP C Ward
Nov 1, 1970·Sabouraudia·S S HippH R Buckley
Jan 1, 1981·Journal of Immunological Methods·W M Hunter, P S Budd
Jan 1, 1984·Acta Radiologica. Oncology·B Nørgaard-PedersenI L Svennekjaer
Jan 1, 1984·Critical Reviews in Clinical Laboratory Sciences·J H Rippey
Jan 1, 1984·European Surgical Research. Europäische Chirurgische Forschung. Recherches Chirurgicales Européennes·K NustadA Berge
Apr 25, 1980·Science·A Rembaum, W J Dreyer
Aug 13, 1982·Journal of Immunological Methods·A RembaumJ Ugelstad
Jan 1, 1982·Biomaterials, Medical Devices, and Artificial Organs·L MarcusS Margel

❮ Previous
Next ❯

Citations

Jul 5, 2001·Journal of Biomaterials Science. Polymer Edition·J L Ortega-Vinuesa, D Bastos-González
Jul 15, 2000·Journal of Biomaterials Science. Polymer Edition·M GadzinowskiC Pichot
Dec 24, 2018·Analytical Chemistry·Teodor Adrian EnacheVictor Constantin Diculescu

❮ Previous
Next ❯

Related Concepts

Related Feeds

Antibodies: Agglutination

Antibody-mediated agglutination is the clumping of cells in the presence of antibody, which binds multiple cells together. This enhances the clearance of pathogens. Find the latest research on antibody-mediated agglutination here.