Adsorption, desorption, and conformational changes of lysozyme from thermosensitive nanomagnetic particles

Journal of Colloid and Interface Science
N ShamimM S Uddin

Abstract

Adsorption of globular protein, lysozyme, on thermosensitive poly(N-isopropylacrylamide) coated nanomagnetic particles was studied at different temperatures and pHs. It was observed that a maximum amount of lysozyme was adsorbed at a temperature above the lower critical solution temperature (LCST) (32 degrees C ) of the polymer and at the isoelectric point (pI=11) of lysozyme. Desorption was carried out using either NaH2PO4 (pH 4) or NaSCN (pH 6) as the desorbing agents. Conformational changes in lysozyme on desorption from nanomagnetic particles was studied by circular dichroism and intrinsic fluorescence spectroscopy. Lysozyme desorbed by NaH2PO4 showed very little conformational changes while lysozyme desorbed by NaSCN showed significant conformational changes, and 87% enzymatic activity was retained in the desorbed enzyme for desorption by NaH2PO4.

References

Sep 7, 2000·Journal of Controlled Release : Official Journal of the Controlled Release Society·M van de WeertD J Crommelin
Jul 21, 2004·Colloids and Surfaces. B, Biointerfaces·Z G PengM S Uddin
Oct 3, 2006·Journal of Colloid and Interface Science·N ShamimM S Uddin

Citations

Sep 30, 2010·Langmuir : the ACS Journal of Surfaces and Colloids·Karina Kubiak-Ossowska, Paul A Mulheran
Jun 5, 2012·Nanoscale Research Letters·Galina A PetkovaVladimír Král
Jul 9, 2013·Journal of Colloid and Interface Science·Jiamin WuYi Tang
Mar 23, 2012·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Jing ZhangYanjun Yang
Sep 24, 2011·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Jun SunYanjun Yang
Feb 20, 2013·Biotechnology Journal·Luís BorlidoJosé Paulo S Farinha
Mar 25, 2016·Biomacromolecules·Nidhi Chandrama DubeyLeonid Ionov
Jan 7, 2015·ACS Applied Materials & Interfaces·Nidhi Chandrama DubeyLeonid Ionov
Aug 16, 2016·Materials Science & Engineering. C, Materials for Biological Applications·Kausar RajarEsra Alveroglu
Sep 16, 2014·Journal of Colloid and Interface Science·Ling LuoSheng Zhang
Mar 5, 2016·Biomedical Materials·Michael C CrossNathan D Gallant
Sep 1, 2015·Chemical Science·Alexander Stephenson-BrownPaula M Mendes
Jan 13, 2018·Physical Chemistry Chemical Physics : PCCP·Luis Pérez-MasArturo Moncho-Jordá

Related Concepts

Oligo(N-isopropylacrylamide)
Acrylamides
Acrylic Resins
Adsorption
Circular Dichroism, Vibrational
Hydrogen-Ion Concentration
Isoelectric Point
Study of Magnetics
Leftose
Polymers

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