Adsorption during Heat Treatment Related to the Thermal Unfolding/Aggregation of beta-Lactoglobulins A and B

Journal of Colloid and Interface Science
Ulla M ElofssonThomas Arnebrant

Abstract

Adsorption onto chromium surfaces during heat treatment (65-68°C) of beta-lactoglobulin A and B in phosphate buffer, pH 6.88, was investigated by in situ ellipsometry. Thermal unfolding and in situ heat-induced aggregation under the same conditions were studied by differential scanning calorimetry and dynamic light scattering, respectively. A lag phase was observed for buildup of the thickness of the adsorbed layer of beta-lactoglobulin A and B at 68°C. The lag period was found to increase with decreasing temperature. The difference in adsorption rate between the variants was found to be in agreement with the aggregation difference in solution, that is, the A variant aggregated more rapidly after a somewhat slower beginning. These results could be related to a difference in the thermal unfolding behavior. By applying a non-two state model the second of two transitions was suggested to involve the activation of the free sulfydryl group. The results indicated that fouling proceeds via attachment of aggregates with an activated free sulfydryl group through an interchange reaction with a disulfide bond in the adsorbed protein layer.

Citations

Feb 26, 2020·Animals : an Open Access Journal From MDPI·Lucio Flavio Macedo MotaAlessio Cecchinato
Oct 30, 1998·Journal of Colloid and Interface Science·M Malmsten
Nov 27, 2008·Langmuir : the ACS Journal of Surfaces and Colloids·Tomoaki HagiwaraHisahiko Watanabe
Mar 27, 1999·Journal of Colloid and Interface Science·H BrunnerH Hoffmann

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