Adsorption, immobilization, and activity of beta-glucosidase on different soil colloids

Journal of Colloid and Interface Science
Jinlong YanAilan Luo

Abstract

For a better understanding of enzyme stabilization and the subsequent catalytic process in a soil environment, the adsorption, immobilization, and activity of beta-glucosidase on various soil colloids from a paddy soil were studied. The calculated parameters maximum adsorption capacity (q(0)) for fine soil colloids ranged from 169.6 to 203.7 microg mg(-1), which was higher than coarse soil colloids in the range of 81.0-94.6 microg mg(-1), but the lower adsorption affinity (K(L)) was found on fine soil colloids. The percentages of beta-glucosidase desorbed from external surfaces of the coarse soil colloids (27.6-28.5%) were higher than those from the fine soil colloids (17.5-20.2%). Beta-glucosidase immobilized on the coarse inorganic and organic soil colloids retained 72.4% and 69.8% of activity, respectively, which indicated the facilitated effect of soil organic matter in the inhibition of enzyme activity. The residual activity for the fine soil clay is 79-81%. After 30 days of storage at 40 degrees C the free beta-glucosidase retained 66.2% of its initial activity, whereas the soil colloidal particle-immobilized enzyme retained 77.1-82.4% of its activity. The half-lives of free beta-glucosidase appeared to be 95.9 and 50.4 d...Continue Reading

References

Jun 18, 2005·Journal of Colloid and Interface Science·Ali Akbar Safari SineganiHossain Shariatmadari
Jul 19, 2005·Colloids and Surfaces. B, Biointerfaces·G V LubarskyR H Bradley
Oct 4, 2005·Colloids and Surfaces. B, Biointerfaces·Qiaoyun HuangPeng Cai

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Citations

Jun 19, 2013·Enzyme and Microbial Technology·Zhu-Ping HanXiao-Ping Dong
May 4, 2015·Applied Biochemistry and Biotechnology·Ashani SamaratungaScott W Pryor
Aug 23, 2016·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Natália G GraebinRafael C Rodrigues
Aug 26, 2014·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Ana M GonçalvesLuís A Passarinha
Sep 1, 2017·Scientific Reports·Samuel Alexander Festing BonnettChris Freeman
Nov 20, 2020·Microorganisms·Folasade K OlagokeCordula Vogel
Nov 26, 2021·Environmental Science and Pollution Research International·Kavita Verma, Jitendra Pandey

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