Abstract
A recent study in our laboratory has identified the potential role of urine-derived alpha-1-microglobulin (alpha-1-m) in mediating Pseudomonas aeruginosa adhesion to polystyrene, while other workers have suggested a possible role of the protein in the immunological response. Due to the ubiquitous presence of alpha-1-m in body fluids, the adsorption of the protein from serum, cerebrospinal fluid, urine and used continuous ambulatory peritoneal dialysis fluid onto polystyrene was investigated. The treated surfaces were sequentially immersed in water and increasingly concentrated isopropanol-water solutions in order to selectively desorb bound proteins on the basis of their binding strength. Sodium dodecyl sulphate-polyacrylamide gel electrophoresis of the wash supernatants showed different protein desorption profiles for each biological fluid, despite the qualitative similarity between the protein composition of the fluids, and highlighted the uptake of alpha-1-m from each fluid to the surface. In the case of urine, the analysis was extended to commercial polyurethane and silicone stents. The ease of desorption of urine-derived alpha-1-m could be correlated with surface hydrophobicity of the stent biomaterial.
References
Jul 19, 1976·Biochimica Et Biophysica Acta·L Tejler, A O Grubb
Jun 1, 1991·Trends in Biotechnology·M Wahlgren, T Arnebrant
Oct 1, 1990·Journal of Clinical Microbiology·M H WilcoxP Williams
Oct 1, 1990·Journal of Biomedical Materials Research·L Hawthorn, G Reid
Apr 1, 1990·Kidney International·A K CheungJ Janatova
Aug 15, 1987·Analytical Biochemistry·M Gottlieb, M Chavko
Oct 1, 1988·Journal of Vascular Surgery·J M Seeger, N Klingman
Sep 1, 1986·Advances in Colloid and Interface Science·W Norde
Dec 1, 1971·Immunochemistry·S Avrameas, T Ternynck
Aug 15, 1970·Nature·U K Laemmli
Aug 1, 1980·Journal of Clinical Pathology·K TakagiT Kawai
Jan 1, 1981·Scandinavian Journal of Immunology·L Lögdberg, B Akerström
Apr 1, 1981·Analytical Biochemistry·W N Burnette
Sep 1, 1984·Infection and Immunity·P E VaudauxU E Nydegger
Feb 1, 1980·Scandinavian Journal of Clinical and Laboratory Investigation·J K KjekshusP Rinde
Jul 1, 1980·Biomaterials·D K GildingJ E Castle
Aug 1, 1994·The Biochemical Journal·C FalkenbergB Akerström
Jan 1, 1995·Electrophoresis·M R BüelerD J Vonderschmitt
Jan 1, 1995·Urological Research·B DussolJ M Verdier
Feb 1, 1993·Molecular Immunology·U R NilssonB Nilsson
Feb 1, 1995·Infection and Immunity·P E VaudauxS L Cooper
Oct 1, 1994·Biomaterials·R Barbucci, A Magnani
Oct 31, 1994·FEBS Letters·D R Flower
Feb 1, 1993·Biomaterials·M E NormanL Illum
Apr 5, 1994·Biotechnology and Bioengineering·P D DrumhellerJ A Hubbell
Citations
May 7, 1998·Journal of Biomedical Materials Research·M SantinM Cannas
Jul 9, 1999·Journal of Biomedical Materials Research·M SantinM Cannas
Mar 17, 2004·Biomaterials·F RenòM Cannas
Aug 14, 1999·Scandinavian Journal of Immunology·M Santin, M Cannas
Sep 21, 2013·Biofouling·Chelsea N ElwoodDirk Lange
Jul 15, 2009·Nature Reviews. Urology·Ben H Chew, Dirk Lange
Jul 17, 2004·Clinica Chimica Acta; International Journal of Clinical Chemistry·Joris Penders, Joris R Delanghe