Adsorption of alpha-chymotrypsin onto mica in laminar flow conditions. Adsorption kinetic constant as a function of tris buffer concentration at pH 8.6

Langmuir : the ACS Journal of Surfaces and Colloids
Elena N Vasina, Philippe Déjardin

Abstract

We examined the adsorption kinetics of alpha-chymotrypsin (pH 8.6, 10(-2) to 0.5 M Tris buffer) on muscovite mica in conditions of laminar flow through a slit. The range of buffer concentrations is between two limits: (i) no adsorption in 1 M Tris and (ii) no desorption in 10(-3) M Tris. Studying the dependence of adsorption kinetics on the wall shear rate leads to the determination of the interfacial adsorption kinetic constant ka and the diffusion coefficient. The obtained value for the diffusion coefficient is close to the one expected from the molecular size of alpha-chymotrypsin. The interfacial adsorption kinetic constant of alpha-chymotrypsin decreases when ionic strength increases, while the initial desorption constant (over a part of all the adsorbed population) shows the contrary. Although alpha-chymotrypsin is almost at its isoelectric point, the effect of ionic strength on the adsorption kinetics suggests the importance of electrostatic interactions between the protein and mica. We observed an increase in the adsorption rate, at a surface coverage near 0.14 microg cm(-2), for adsorption in 10(-2) M Tris and the low wall shear rates (<300 s(-1)). This change in the adsorption rate suggests a structural transition, th...Continue Reading

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Citations

Apr 9, 2013·Colloids and Surfaces. B, Biointerfaces·Elena N Vasina, Philippe Déjardin
Apr 14, 2011·Microbes and Environments·Kazutoshi SaekiMasao Sakai
Feb 4, 2014·Advances in Colloid and Interface Science·Mark KastantinDaniel K Schwartz
Oct 13, 2012·Langmuir : the ACS Journal of Surfaces and Colloids·Maria Dąbkowska, Zbigniew Adamczyk
Sep 27, 2012·Colloids and Surfaces. B, Biointerfaces·Maria DąbkowskaMarta Kujda
Oct 18, 2011·Journal of Colloid and Interface Science·Maria Dąbkowska, Zbigniew Adamczyk
May 31, 2011·Advances in Colloid and Interface Science·Z AdamczykM Zaucha
Feb 15, 2011·Journal of Colloid and Interface Science·Z AdamczykM Sadowska
Nov 29, 2015·Colloids and Surfaces. B, Biointerfaces·Marta KujdaEwa Kowalska
Jan 26, 2010·Journal of Colloid and Interface Science·Sunhyung KimSeung Jong Lee
Nov 17, 2009·Colloids and Surfaces. B, Biointerfaces·Sylvie NoinvillePhilippe Déjardin
Feb 3, 2009·Colloids and Surfaces. B, Biointerfaces·Sandrine DemanècheHervé Quiquampoix
Sep 15, 2015·Chemistry : a European Journal·Susobhan ChoudhurySamir Kumar Pal
Mar 28, 2007·Chemphyschem : a European Journal of Chemical Physics and Physical Chemistry·Michael RabeStefan Seeger
Feb 14, 2015·Colloids and Surfaces. B, Biointerfaces·Marta KujdaKamila Sofińska
May 13, 2011·Journal of Colloid and Interface Science·Maria ZauchaJakub Barbasz
Feb 8, 2011·Advances in Colloid and Interface Science·Michael RabeStefan Seeger
Oct 10, 2008·The Journal of Physical Chemistry. B·Michael RabeStefan Seeger
Apr 7, 2010·Langmuir : the ACS Journal of Surfaces and Colloids·Zbigniew AdamczykMaria Zembala
Jul 16, 2019·Advances in Colloid and Interface Science·Diego CoglitoreSebastien Balme
Dec 16, 2010·Langmuir : the ACS Journal of Surfaces and Colloids·Monika Wasilewska, Zbigniew Adamczyk
Aug 6, 2019·Langmuir : the ACS Journal of Surfaces and Colloids·Changan HuangfuXiaohua Lu
Jul 22, 2005·Langmuir : the ACS Journal of Surfaces and Colloids·Philippe DéjardinVitaly I Volkov
Aug 24, 2005·Langmuir : the ACS Journal of Surfaces and Colloids·Hyong-Jun KimJinsang Kim

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