Adsorption of an endoglucanase from the hyperthermophilic Pyrococcus furiosus on hydrophobic (polystyrene) and hydrophilic (silica) surfaces increases protein heat stability

Langmuir : the ACS Journal of Surfaces and Colloids
Sotiris KoutsopoulosWillem Norde

Abstract

The interaction of an endoglucanase from the hyperthermophilic microorganism Pyrococcus furiosus with two types of surfaces, that is, hydrophobic polystyrene and hydrophilic silica, was investigated, and the adsorption isotherms were determined. The adsorbed hyperthermostable enzyme did not undergo loss of biological activity. A model was proposed for the mechanism of interaction of the enzyme with the surface based on the shape of the adsorption isotherm, the morphological characteristics of the enzyme, and the thermodynamic parameters of the system. The enzyme was irreversibly immobilized at the solid/liquid interface even at high temperatures, and most interestingly, it acquired further heat stabilization upon adsorption. The denaturation temperature increased from 108 degrees C in solution to 116 degrees C upon adsorption on hydrophilic silica particles. Adsorption on the hydrophobic polystyrene surface even shifted the denaturation temperature to 135 degrees C, the most extreme experimentally determined protein denaturation temperature ever reported. Maintenance of the biological function particularly at high temperatures is important for the development of solid substrate immobilized enzymes for applications in biocatalys...Continue Reading

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Citations

Oct 20, 2012·Biomedical Microdevices·Christopher R PhaneufCraig R Forest
Feb 23, 2010·Biochemical and Biophysical Research Communications·Toru MizukiToru Maekawa
Oct 20, 2007·The FEBS Journal·Sotirios KoutsopoulosWillem Norde
Nov 13, 2008·Macromolecular Bioscience·Shunsheng Cao, Bailing Liu
Jan 4, 2010·Macromolecular Rapid Communications·Nemanja MiletićKatja Loos
Oct 1, 2011·Colloids and Surfaces. B, Biointerfaces·Dong-Hao ZhangXiao-Bing Li
Oct 23, 2013·Journal of Biomaterials Applications·Abeer JabaiahSzu-Wen Wang
Aug 20, 2009·Langmuir : the ACS Journal of Surfaces and Colloids·Zhe AnYan Wang

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