Adsorption of bovine serum albumin on polyelectrolyte-coated glass substrates: applications to colloidal lithography

Colloids and Surfaces. B, Biointerfaces
Y H Miao, L E Helseth

Abstract

The adsorption of bovine serum albumin (BSA) labeled with fluorescein isothiocyanate (FITC) on polyelectrolyte-coated glass substrates was investigated using fluorescence microscopy. Glass substrates may inhibit adsorption of proteins due to electrostatic repulsion. However, when the substrate is modified with a thin film of positively charged polyelectrolytes, proteins can be adsorbed due to the attractive electrostatic interactions. In this study, poly(allylamine-hydrochloride) (PAH) molecules, which have positively charged amino groups at pH 7, were used to generate a positively charged layer on the glass substrate. A surfactant, sodium dodecyl sulphate (SDS), was used to alter the glass-protein interaction and subsequently modulate the coverage of adsorbed protein. We applied this technique to control the heterogeneously charged microscopic patterns of biomolecules created when the adsorption of protein is done in conjunction with colloidal lithography.

References

Jul 16, 1992·Nature·X M He, D C Carter
Jun 1, 2005·Langmuir : the ACS Journal of Surfaces and Colloids·Fung Ling Yap, Yong Zhang
Aug 24, 2005·Langmuir : the ACS Journal of Surfaces and Colloids·Karen L Christman, Heather D Maynard
Sep 21, 2005·Langmuir : the ACS Journal of Surfaces and Colloids·Yuguang Cai, Benjamin M Ocko
May 31, 2006·Langmuir : the ACS Journal of Surfaces and Colloids·Nikhil D KalyankarLane Gilchrist
Jun 30, 2006·Colloids and Surfaces. B, Biointerfaces·Nuzhat Gull Kabir-ud-Din
Feb 13, 2007·Biomacromolecules·Dan WuYujun Feng
Jul 10, 2007·Langmuir : the ACS Journal of Surfaces and Colloids·D L GengL E Helseth

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Citations

Dec 9, 2009·Colloids and Surfaces. B, Biointerfaces·Elena PrimoracDavor Kovacević
Jul 1, 2009·Colloids and Surfaces. B, Biointerfaces·Chao XuShenglian Luo
Jul 26, 2015·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Z W ZhongH L Tan
Apr 15, 2014·The Journal of Physical Chemistry. B·Tanmay BeraJiyu Fang
Oct 5, 2011·Journal of Biomaterials Science. Polymer Edition·Maria BulwanMaria Nowakowska
Mar 30, 2012·Journal of Biomaterials Applications·Andrea NolteRumen Krastev
Aug 3, 2010·Langmuir : the ACS Journal of Surfaces and Colloids·Rubens A SilvaPaul L Dubin

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