Adsorption of chain type-specific ABO antibodies on Sepharose-linked A and B tetrasaccharides

Transfusion
Linda LindbergJan Holgersson

Abstract

Antigen-specific removal of anti-A and anti-B on immunoadsorption columns carrying the blood group A and B trisaccharides is one important component of some protocols used in ABO-incompatible organ transplantation. Because ABO antibodies exist requiring parts of the core saccharide chain for binding, the anti-A and -B-binding capacity of individual and combined, Sepharose-linked Types 1 through 4 A and B tetrasaccharides with that of the A and B trisaccharides was compared. Sepharose-linked A and B tri- and tetrasaccharides were used to adsorb anti-A and -B from pooled blood group O serum. Remaining chain type-specific anti-A and -B were detected and quantified in enzyme-linked immunosorbent assays using wells coated with neoglycoproteins or recombinant mucins carrying A and B determinants on defined core saccharide chains. Significantly more anti-A Type 3- and 4-specific immunoglobulin (Ig)G remained after adsorption on the A trisaccharide and the A Type 1 and A Type 2 tetrasaccharide than after adsorption on the A Types 3 and 4 tetrasaccharides. Selective adsorption of chain type-specific IgG anti-B was detected on Sepharose-linked B tetrasaccharides. In contrast, there were no chain type-specific IgM anti-A or -B. A combinat...Continue Reading

References

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Citations

Jan 13, 2016·Glycobiology·Jeffrey C Gildersleeve, W Shea Wright
Apr 23, 2013·American Journal of Hematology·Shruti ChaturvediKeith R McCrae
Sep 2, 2015·Nature Reviews. Nephrology·Georg A BöhmigThomas Wekerle
Sep 5, 2015·Oncotarget·Saddam M MuthanaJeffrey C Gildersleeve
Feb 22, 2017·The International Journal of Artificial Organs·Vladislav SemakViktoria Weber
Jun 9, 2021·American Journal of Transplantation : Official Journal of the American Society of Transplantation and the American Society of Transplant Surgeons·Andrew BentallSimon Ball

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