Adsorption of collagenase to particulate titanium: a possible mechanism for collagenase localization in periprosthetic tissue

Journal of Applied Biomaterials : an Official Journal of the Society for Biomaterials
K R KaneA B Swanson

Abstract

Osteolysis is a central feature of aseptic loosening of orthopaedic joint prostheses. This destructive process is believed to result from phagocytosis of implant wear debris by periprosthetic and synovial macrophages and the subsequent release of proinflammatory mediators, including collagenase. Isolated murine macrophages were cultured in vitro with particulate titanium in order to explore the mechanism of macrophage activation by particulate wear debris. The results, in which the amount of secreted, soluble collagenase in culture supernatants was inversely proportional to titanium concentration, suggested that titanium strongly adsorbed secreted collagenase. This inference was confirmed by direct binding assays in which particulate titanium coated with adsorbed collagenase bound an alkaline phosphatase conjugated anti-collagenase antibody, but not a conjugated anti-IgG antibody. Adsorption of collagenase was not influenced by preincubation of titanium particles with albumin. The adsorbed collagenase remained enzymatically active as indicated by its ability to hydrolyze a synthetic peptide substrate. These results demonstrate that particulate titanium stimulates collagenase production by macrophages and then strongly adsorbs t...Continue Reading

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Citations

Jun 1, 2002·Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society·Phillip L PalmbosJoy D Bonnema
Feb 5, 1999·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·B NawrockiJ J Adnet
May 1, 1995·The Journal of Hand Surgery·D H DeHeerA B Swanson
May 19, 2017·Journal of Biological Inorganic Chemistry : JBIC : a Publication of the Society of Biological Inorganic Chemistry·Justin Paul Curtin, Minji Wang
Apr 16, 2002·Clinical Orthopaedics and Related Research·Douglas B FreelsWilliam G Ward
Sep 28, 2000·Journal of Biomedical Materials Research·M AharizP Courtois

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