Adsorption of lysozyme, beta-casein and their layer-by-layer formation on hydrophilic surfaces: Effect of ionic strength

Colloids and Surfaces. B, Biointerfaces
Maria LundinMark W Rutland

Abstract

The adsorbed amount and layer structure of lysozyme, beta-casein and mixed layers of the two proteins were studied on hydrophilic silica and quartz surfaces using the following techniques: ellipsometry, quartz crystal microbalance with dissipation monitoring (QCM-D) and total internal reflection fluorescence (TIRF). Particular emphasis was put on the effect of solution ionic strength on the layer formation. Both lysozyme and beta-casein showed a higher affinity for the silica surface when adsorbed from a solution of low ionic strength even though beta-casein and silica are negatively charged at the pH used. No beta-casein remained adsorbed after rinsing with a 150mM buffer solution. The adsorbed amount of lysozyme on silica exceeded a monolayer coverage irrespective of the solution conditions and displayed a rigid structure. beta-Casein forms more than a single layer on pre-adsorbed lysozyme; an inner flat layer and an outer layer with an extended structure, which largely desorbs on rinsing. The build-up through sequential adsorption of lysozyme and beta-casein is favoured at intermediate and high ionic strength. The total adsorbed amount increased slightly with each deposition cycle and the mixed lysozyme/beta-casein layers co...Continue Reading

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Citations

Sep 30, 2010·Langmuir : the ACS Journal of Surfaces and Colloids·Karina Kubiak-Ossowska, Paul A Mulheran
Feb 20, 2016·Colloids and Surfaces. B, Biointerfaces·Yan LiYihong Gong
Nov 1, 2012·Artificial Cells, Nanomedicine, and Biotechnology·Kazım Köse, Adil Denizli
Oct 22, 2011·Journal of Colloid and Interface Science·M LundinI Odnevall Wallinder
Aug 18, 2012·Journal of Molecular Recognition : JMR·Robert E Speight, Matthew A Cooper
Aug 1, 2012·Carbohydrate Polymers·Marta WestwoodVictor J Morris
Feb 15, 2014·Colloids and Surfaces. B, Biointerfaces·Mahdi Dargahi, Sasha Omanovic
Jul 29, 2016·Langmuir : the ACS Journal of Surfaces and Colloids·Dharana JayawardaneXiubo Zhao
Jul 13, 2012·Langmuir : the ACS Journal of Surfaces and Colloids·Chris H J EversMarie Skepö
May 28, 2019·Polymers·Lilianna Szyk-WarszyńskaPiotr Warszyński
Nov 21, 2012·Journal of Physics. Condensed Matter : an Institute of Physics Journal·Yohko F Yano
Feb 3, 2021·International Journal of Molecular Sciences·Paulina KomorekBarbara Jachimska
Mar 31, 2021·Journal of Chemical Theory and Computation·Martin ReinhardtRebecca C Wade
Jul 24, 2013·Langmuir : the ACS Journal of Surfaces and Colloids·Fabian MederKurosch Rezwan

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