PMID: 3753757Jan 1, 1986Paper

Adsorption of natural lung surfactant and phospholipid extracts related to tubular myelin formation

Pediatric Research
R H NotterD L Shapiro

Abstract

The microstructure of aqueous dispersions of pulmonary surfactant lipids extracted from bovine lung lavage was investigated by electron microscopic analysis. Following organic solvent extraction (chloroform/methanol) from natural lung surfactant, the mixed lipids (CLL), with 1% residual protein, were dispersed in water by two techniques, probe sonication at 4 degrees C and mechanical vortexing. Surface pressure-time adsorption isotherms were defined for the CLL dispersions, followed by staining with tannic acid, uranyl acetate, and lead citrate for electron microscopy of microstructure. CLL extract dispersions adsorbed in seconds to surface pressures near 45 dynes/cm (surface tension 25 dynes/cm) at low concentrations less than or equal to 0.25 mg/ml after dispersion by sonication of 4 degrees C and by mechanical vortexing. Ultrastructurally, the CLL dispersions were somewhat heterogeneous, but large thin-walled phospholipid vesicles, both intact and fragmented, predominated. No tubular myelin was formed. By contrast, natural lung surfactant (LS) from bronchoalveolar lavage had characteristic regions of tubular myelin when it adsorbed well at low concentrations (less than or equal to 0.25 mg phospholipid/ml). When divalent cati...Continue Reading

Citations

Oct 17, 1987·Biochimica Et Biophysica Acta·S H YuF Possmayer
Apr 26, 1989·Biochimica Et Biophysica Acta·J ChungF Possmayer
Jan 1, 1991·Chemistry and Physics of Lipids·A R VenkitaramanR H Notter
Feb 14, 2002·Chemistry and Physics of Lipids·Robert H NotterB A Holm
Feb 22, 2001·Annual Review of Physiology·S Hawgood, F R Poulain
Feb 16, 2005·Annual Review of Physiology·Paul Dietl, Thomas Haller
Nov 14, 1997·American Journal of Respiratory and Critical Care Medicine·Z WangR H Notter
Jul 10, 1999·Pediatric Research·B A HolmR H Notter
May 1, 1988·Experimental Lung Research·B Robertson, B Lachmann
Jul 14, 2012·Yakugaku zasshi : Journal of the Pharmaceutical Society of Japan·Hiromichi Nakahara
Dec 5, 2006·Seminars in Perinatology·Mikko Hallman, Ritva Haataja
May 12, 2005·American Journal of Physiology. Lung Cellular and Molecular Physiology·Zhengdong WangRobert H Notter
Jan 17, 2002·American Journal of Physiology. Lung Cellular and Molecular Physiology·David M KingRobert H Notter
Sep 26, 2001·Protein Expression and Purification·J E BaatzR H Notter
Jan 1, 1995·Life Sciences·L R McLean, J E Lewis

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