Adsorptive immobilization of agro-industrially produced crude laccase on various micro-biochars and degradation of diclofenac

The Science of the Total Environment
Linson LonappanR Y Surampalli

Abstract

Although enzymes are gifted with unique and unprecedented catalytic activity and selectivity over a wide range of pollutants, still their stability related issues often hinder their application in real environmental conditions. In this study, agro-industrially produced crude laccase was concentrated using ultrafiltration. Crude laccase was immobilized on pine wood (BC-PW), pig manure (BC-PM) and almond shell (BC-AS) biochar microparticles. Immobilization of laccase was investigated at various laccase activities on micro-biochars and the release (desorption) of the enzyme has been studied. It was observed that for all the biochars, as the initial concentration of laccase increased in the crude solution, the binding capacity and as result immobilization efficiency also increased. BC-PM was found to be the most effective (31.4 ± 3.1 U g-1) at 10 U mL-1 of enzyme activity followed by BC-AS (24.3 ± 4.8 U g-1) and BC-PW (14.58 ± 3.3 U g-1). In addition, the biochars were functionalized with citric acid for possible surface modifications and the effect of biochars for the adsorption of enzymes has been investigated. Isotherm studies of enzyme loading onto biochar established homogeneous monolayer adsorption as the major mechanism. The...Continue Reading

Citations

May 28, 2019·Topics in Current Chemistry·Efaq NomanBalkis A Talip
Jan 14, 2021·Biotechnology & Genetic Engineering Reviews·Dajun RenXiaoqing Zhang
Nov 20, 2020·The Science of the Total Environment·Willis GwenziMunyaradzi Mtisi
Mar 19, 2021·Marine Pollution Bulletin·Rozita Madadi, Kai Bester
Jun 23, 2021·Journal of Hazardous Materials·Palanivel SathishkumarFeng Long Gu

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