Adult bone marrow-derived cells do not acquire functional attributes of cardiomyocytes when transplanted into peri-infarct myocardium.

Molecular Therapy : the Journal of the American Society of Gene Therapy
John A ScherschelMichael Rubart

Abstract

The cardiomyogenic potential of adult bone marrow (BM) cells after being directly transplanted into the ischemically injured heart remains a controversial issue. In this study, we investigated the ability of transplanted BM cells to develop intracellular calcium ([Ca(2+)](i)) transients in response to membrane depolarization in situ. Low-density mononuclear (LDM) BM cells, c-kit-enriched (c-kit(enr)) BM cells, and highly enriched lin(-) c-kit(+) BM cells were obtained from adult transgenic mice ubiquitously expressing enhanced green fluorescent protein (EGFP), and injected into peri-infarct myocardiums of nontransgenic mice. After 9-10 days the mice were killed, and the hearts were removed, perfused in Langendorff mode, loaded with the calcium-sensitive fluorophore rhod-2, and subjected to two-photon laser scanning fluorescence microscopy (TPLSM) to monitor action potential-induced [Ca(2+)](i) transients in EGFP-expressing donor-derived cells and non-expressing host cardiomyocytes. Whereas spontaneous and electrically evoked [Ca(2+)](i) transients were found to occur synchronously in host cardiomyocytes along the graft-host border and in areas remote from the infarct, they were absent in all of the >3,000 imaged BM-derived cell...Continue Reading

References

May 5, 1997·FEBS Letters·M OkabeY Nishimune
Oct 20, 1999·Nature Neuroscience·F HelmchenD W Tank
Nov 2, 1999·Microscopy Research and Technique·R GauderonC J Sheppard
Apr 5, 2001·Nature·D OrlicP Anversa
Jun 8, 2001·The Journal of Clinical Investigation·K A JacksonM A Goodell
Feb 14, 2003·American Journal of Physiology. Cell Physiology·Michael RubartLoren J Field
May 6, 2003·Circulation Research·Michael RubartLoren J Field
Jun 12, 2003·Annals of the New York Academy of Sciences·Donald Orlic
Sep 11, 2004·Circulation Research·Francisco Fernández-AvilésAna Sánchez
Dec 14, 2004·Circulation Research·Michael Rubart
Dec 29, 2004·Cardiovascular Research·Alexander DetenHeinz-Gerd Zimmer
Mar 9, 2005·Circulation·Jane A Leopold, Elliott M Antman
Jul 9, 2005·Nature Biotechnology·Michael A Laflamme, Charles E Murry
Nov 25, 2005·Circulation Research·Hidehiro NakajimaLoren J Field
Feb 8, 2006·Annual Review of Physiology·Michael Rubart, Loren J Field
Nov 23, 2006·Proceedings of the National Academy of Sciences of the United States of America·Sandra B HaudekMark L Entman
Oct 30, 2007·Proceedings of the National Academy of Sciences of the United States of America·Marcello RotaPiero Anversa

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Citations

Mar 12, 2009·Molecular Therapy : the Journal of the American Society of Gene Therapy·Philippe Menasche
Feb 19, 2009·Molecular Therapy : the Journal of the American Society of Gene Therapy·Susanne ProkschPhilippe Menasché
Oct 23, 2009·Proceedings of the National Academy of Sciences of the United States of America·Hannah SongPeter W Zandstra
May 7, 2009·Antioxidants & Redox Signaling·Ebo D de Muinck
Jan 12, 2010·Annual Review of Medicine·Stefan Janssens
Nov 8, 2008·Circulation Research·Hans ReineckeMichael A Laflamme
Feb 20, 2010·Circulation Research·John V TerrovitisEduardo Marbán
Apr 8, 2009·Circulation·Ester Macia, Penelope A Boyden
Dec 23, 2009·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·Yan ZhouJiang Gu
Aug 1, 2012·Biologics : Targets & Therapy·Steve Elliott, Angus M Sinclair
Mar 9, 2012·Vascular Health and Risk Management·Jane Hoover-Plow, Yanqing Gong
Jul 26, 2011·Comptes rendus biologies·Philippe Menasché
Mar 5, 2011·Advanced Drug Delivery Reviews·Zhaoyang YeWensong Tan
Feb 24, 2009·The Annals of Thoracic Surgery·Courtney N ZellerDaniel R Meldrum
Mar 12, 2010·Journal of Tissue Engineering and Regenerative Medicine·Matthew J WebberJon W Lomasney
Jul 21, 2010·Journal of Molecular and Cellular Cardiology·Sveva BolliniPaul R Riley
Jan 12, 2010·Journal of Molecular and Cellular Cardiology·Liliana GrajalesDavid L Geenen
Jan 13, 2015·Cellular and Molecular Life Sciences : CMLS·Patrizia NigroGiulio Pompilio
Dec 19, 2014·Frontiers in Physiology·Xiao-Long Lu, Michael Rubart
Nov 5, 2014·Frontiers in Cell and Developmental Biology·Cécile DromardValerie Planat-Benard
Dec 25, 2008·Expert Review of Cardiovascular Therapy·Kenneth R Boheler
Aug 16, 2018·Frontiers in Physiology·Jason S JonesNozomi Nishimura
Jan 11, 2020·Stem Cell Research & Therapy·Khawaja Husnain Haider, Beatrice Aramini
Jun 12, 2021·Medicinal Research Reviews·Hanne BeliënAnnelies Bronckaers

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