Nov 9, 2018

Adult Canine Intestinal Derived Organoids: A Novel In Vitro System for Translational Research in Comparative Gastroenterology

BioRxiv : the Preprint Server for Biology
Lawrance ChandraKarin Allenspach

Abstract

Background: Large animal models, such as the dog, are increasingly being used over rodent models for studying naturally occurring diseases including gastrointestinal (GI) disorders. Dogs share similar environmental, genomic, anatomical, and intestinal physiologic features with humans. To bridge the gap between currently used animal models (e.g. mouse) and humans, and expand the translational potential of the dog model, we developed a three dimensional (3D) canine GI organoid (enteroid and colonoid) system. Organoids have recently gained interest in translational research as this model system better recapitulates the physiological and molecular features of the tissue environment in comparison with two-dimensional cultures. Results: Organoids were propagated from isolation of adult intestinal stem cells (ISC) from whole jejunal tissue as well as endoscopically obtained duodenal, ileal and colonic biopsy samples of healthy dogs and GI cases, including inflammatory bowel disease (IBD) and intestinal carcinomas. Intestinal organoids were comprehensively characterized using histology, immunohistochemistry, RNA in situ hybridization and transmission electron microscopy, and organoids mimicked the in vivo tissue environment. Physiologi...Continue Reading

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Mentioned in this Paper

Exosomes
Metabolic Process, Cellular
Gastroenterology (Field)
In Vivo
Pathogenic Aspects
Cystic-fibrosis Membrane-conductance-regulating Protein Activity
OMI _ Imaging Order
Pathogenesis
Immunohistochemistry
Histology Procedure

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