PMID: 7034289Jan 1, 1981Paper

Adult mammalian cardiac muscle cells in culture

Tissue & Cell
A C Nag, M Cheng

Abstract

Adult rat cardiac muscle cells were isolated from the ventricle by a retrograde perfusion technique through the aorta (Nag and Zak, 1979). These single, isolated cardiac muscle cells were cultured for 4 weeks. Throughout the culture period, a small number of muscle cells retained their cylindrical shape, while the rest exhibited alterations in shape and size assuming a flattened body of irregular shape with pseudopodia-like processes and thereby resembling embryonic/neonatal cardiac muscle cells in culture. Transmission electron microscopy revealed that the cylindrical muscle cells contained compactly arranged myofibrils and cellular organelles, similar to those of freshly isolated and in vivo cells. A few irregularly shaped cardiac muscle cells were similar to the cylindrical cells in their internal structural organization. Most of the irregular cells exhibited less myofibrillar content than that of the freshly dissociated and in vivo cells. Myofibrils in the irregular cells were widely spaced and myofilament of some of the myofibrils were loosely bunched. In addition, scattered patches of myofibrils and free myofilaments were observed in many of these cells. The internal structural organization of these irregularly shaped car...Continue Reading

References

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Citations

Jun 13, 1998·The Anatomical Record·D G MatzJ C Oberpriller
Dec 1, 1989·In Vitro Cellular & Developmental Biology : Journal of the Tissue Culture Association·W C ClaycombR L Moses
Apr 1, 1987·In Vitro Cellular & Developmental Biology : Journal of the Tissue Culture Association·A C Nag, M Cheng
May 1, 1990·In Vitro Cellular & Developmental Biology : Journal of the Tissue Culture Association·A C NagJ R Kosiur
Jan 29, 1986·Brain Research·C J Hassall, G Burnstock
Jan 1, 1985·Tissue & Cell·M S Forbes, N Sperelakis
Jan 1, 1989·Tissue & Cell·J M TateJ C Oberpriller
Apr 1, 1999·Annual Review of Physiology·G ThibaultR Garcia
Jul 28, 2006·PLoS Biology·Ching-Ling LienMark T Keating
Jul 5, 2011·Journal of Molecular and Cellular Cardiology·William E LouchBeata M Wolska
Jun 15, 2010·Journal of Molecular and Cellular Cardiology·Karin HammerPeter Lipp
Jan 1, 1988·Annals of the New York Academy of Sciences·M BorgersG Vandeplassche
Jun 1, 1982·The American Journal of Anatomy·R L Moses, W C Claycomb
Oct 1, 1984·The American Journal of Anatomy·R L Moses, W C Claycomb
Mar 1, 1989·Circulation Research·L B Bugaisky, R Zak
Nov 17, 2015·Biochemistry. Biokhimii︠a︡·N B BildyugG P Pinaev
Jun 28, 2013·American Journal of Physiology. Cell Physiology·Michael FineDonald W Hilgemann
Apr 1, 2000·Laboratory Investigation; a Journal of Technical Methods and Pathology·M OkunoK Yamauchi-Takihara
Sep 1, 1991·The American Journal of Physiology·W A ClarkR S Decker

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