Adult neural precursor cells form connexin-dependent networks that improve their survival

Neuroreport
Ajaya RavellaEric Herlenius

Abstract

Establishment of cellular networks and calcium homeostasis are essential for embryonic stem cell proliferation and differentiation. We also hypothesized that adult neural progenitor cells form functional cellular networks relevant for their development. We isolated neuronal progenitor cells from the subventricular zone of 5-week-old mice to investigate the role of gap junctions, calcium homeostasis, and cellular networks in cell differentiation and survival. Western blotting and reverse transcription-PCR showed that the cells expressed the gap junction components connexin 26, 36, 43, and 45, and that expression of connexin 43 increased in early (8 days) differentiated cells. Transmission electron microscopy and immunocytochemistry also indicated that gap junctions were present. Scrape-loading experiments showed dye transfer between cells that could be prevented by gapjunction blockers; thus, functional intercellular gap junctions had been established. However, dye transfer was four times stronger in differentiated cultures, correlating with the increased connexin 43 expression. During time-lapse calcium imaging, both differentiated and undifferentiated cultures showed spontaneous calcium activity that was reduced by gap junctio...Continue Reading

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Citations

Feb 15, 2017·Reviews in the Neurosciences·Elena A PozhilenkovaAlla B Salmina
Nov 14, 2019·Nature Neuroscience·Erik JungFrank Winkler
Jan 16, 2021·International Journal of Molecular Sciences·Alejandro HerreraEsperanza R Matarredona

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Methods Mentioned

BETA
Transfection
reverse transcription-PCR
PCR
Transmission electron microscopy
light microscopy

Software Mentioned

ImageJ
MATLAB

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