Adult rat retinal ganglion cells and glia can be printed by piezoelectric inkjet printing

Biofabrication
Barbara LorberKeith R G Martin

Abstract

We have investigated whether inkjet printing technology can be extended to print cells of the adult rat central nervous system (CNS), retinal ganglion cells (RGC) and glia, and the effects on survival and growth of these cells in culture, which is an important step in the development of tissue grafts for regenerative medicine, and may aid in the cure of blindness. We observed that RGC and glia can be successfully printed using a piezoelectric printer. Whilst inkjet printing reduced the cell population due to sedimentation within the printing system, imaging of the printhead nozzle, which is the area where the cells experience the greatest shear stress and rate, confirmed that there was no evidence of destruction or even significant distortion of the cells during jet ejection and drop formation. Importantly, the viability of the cells was not affected by the printing process. When we cultured the same number of printed and non-printed RGC/glial cells, there was no significant difference in cell survival and RGC neurite outgrowth. In addition, use of a glial substrate significantly increased RGC neurite outgrowth, and this effect was retained when the cells had been printed. In conclusion, printing of RGC and glia using a piezoel...Continue Reading

References

Jul 23, 1999·Survey of Ophthalmology·J G NichollsM Wintzer
Jun 15, 2004·Biomaterials·Tao XuThomas Boland
Jan 18, 2006·Tissue Engineering·Makoto NakamuraSetsuo Takatani
Oct 16, 2007·Biomaterials·Rachel E SaundersBrian Derby
Nov 1, 2007·Brain : a Journal of Neurology·Adrienne MüllerDietmar Fischer
Apr 26, 2008·Molecular & Cellular Proteomics : MCP·Stefanie M HauckMarius Ueffing
Jun 12, 2008·Tissue Engineering. Part B, Reviews·Tamer A E AhmedMax Hincke
Sep 2, 2008·Tissue Engineering. Part a·Tao XuJames J Yoo
Apr 22, 2009·Journal of Neuroscience Research·Barbara LorberAnn Logan
Jul 1, 2010·Biotechnology and Bioengineering·Xiaofeng CuiThomas Boland
Jul 14, 2010·Journal of Controlled Release : Official Journal of the Controlled Release Society·Alex J T HyattKeith R Martin
Sep 3, 2010·Biofabrication·Shabnam ParsaKaren C Cheung
Aug 27, 2011·Neurobiology of Disease·Barbara LorberKeith R Martin
Jan 26, 2012·Neurobiology of Disease·Marco LeibingerDietmar Fischer
May 26, 2012·Biotechnology and Bioengineering·Daljeet ChahalKaren C Cheung
Oct 19, 2012·Biofabrication·Shuichi YamaguchiKeisuke Morishima
Feb 3, 2013·Biomaterials Science·Cameron J FerrisMarc In Het Panhuis

❮ Previous
Next ❯

Citations

Feb 10, 2016·Trends in Molecular Medicine·Ashkan Shafiee, Anthony Atala
Apr 18, 2016·Drug Discovery Today·Ibrahim T OzbolatVeli Ozbolat
Apr 6, 2016·Current Opinion in Ophthalmology·Barbara LorberKeith R Martin
Apr 27, 2016·Biomaterials Research·Bon Kang GuChun-Ho Kim
May 20, 2016·Pharmaceutical Research·Mohamed A AlhnanBasel Arafat
Mar 31, 2015·PloS One·Barbara LorberKeith R Martin
Sep 21, 2016·Journal of Translational Medicine·Jipeng LiHuifang Zhou
Dec 21, 2016·Advanced Healthcare Materials·Haitao CuiLijie Grace Zhang
Sep 21, 2017·International Journal of Molecular Sciences·Tien-Chun YangShih-Jen Chen
May 9, 2018·Tissue Engineering. Part a·Tetsuya ImamuraOsamu Ishizuka
Dec 6, 2017·Frontiers in Bioengineering and Biotechnology·Michaela Thomas, Stephanie M Willerth
Dec 20, 2014·Journal of Laboratory Automation·Lena SchoberThomas Bauernhansl
Jan 25, 2019·Expert Opinion on Drug Discovery·Sina Azizi MachekposhtiRoger J Narayan
Mar 14, 2019·Journal of Tissue Engineering and Regenerative Medicine·Sindhuja D EswaramoorthySubha N Rath
Apr 13, 2019·International Journal of Molecular Sciences·Shirley Suet Lee DingPooi Ling Mok
Mar 1, 2016·Biofabrication·J L CurleyD B Chrisey
Mar 2, 2016·Biofabrication·Christopher TsePatrick J Smith
Mar 2, 2016·Biofabrication·Ahu Arslan-YildizUtkan Demirci
Sep 24, 2016·Biofabrication·Katja HölzlAleksandr Ovsianikov
Dec 6, 2017·The Journal of Bone and Joint Surgery. American Volume·Matthew J Allen
Oct 9, 2018·Frontiers in Neuroscience·Oriane RabesandratanaGaël Orieux
Jun 19, 2019·Journal of Ocular Pharmacology and Therapeutics : the Official Journal of the Association for Ocular Pharmacology and Therapeutics·Owen S FentonRobert Langer
Aug 20, 2019·Advanced Materials·Paulina Nuñez BernalRiccardo Levato
Feb 10, 2016·Tissue Engineering. Part B, Reviews·Nick A SearsElizabeth Cosgriff-Hernandez
Nov 22, 2016·Annals of the New York Academy of Sciences·Brad J TricomiDavid T Corr
Apr 25, 2018·Journal of Tissue Engineering·Parker E LudwigJorge M Zuniga
Nov 18, 2018·Journal of Cellular Physiology·Rahul MittalVasanti M Jhaveri
Jul 25, 2019·Frontiers in Bioengineering and Biotechnology·Dilara Goksu TamayVasif Hasirci
Dec 21, 2016·Journal of Materials Chemistry. B, Materials for Biology and Medicine·Ju Young ParkDong-Woo Cho
Oct 12, 2018·Journal of Tissue Engineering·Patrick RiderMike Barbeck
Dec 7, 2018·Current Pharmaceutical Design·Musarrat H WarsiSaba Khan
Jan 16, 2020·Polymers·Angelika ZaszczynskaArkadiusz Gradys
Oct 5, 2019·Materials·Natasha Antill-O'BrienCathal D O'Connell
Aug 8, 2019·Cells·Valentina FantiniCristina Cereda
May 23, 2018·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·Gregor SkeldonWenmiao Shu
Jul 25, 2019·Current Pharmaceutical Design·Anish ChandekarGaurav Gupta
Jan 1, 2019·International Journal of Smart and Nano Materials·Timothy J EsworthyLijie Grace Zhang
Oct 6, 2020·Macromolecular Bioscience·Jaideep AdhikariProsenjit Saha
Nov 18, 2020·Advanced Healthcare Materials·Melissa CadenaSteven A Sloan
Mar 4, 2021·Pharmaceutics·Sandra Ruiz-AlonsoJosé Luis Pedraz
Mar 7, 2021·International Journal of Pharmaceutics·Susannah Elizabeth EvansRonan Daly

❮ Previous
Next ❯

Related Concepts

Related Feeds

Allogenic & Autologous Therapies

Allogenic therapies are generated in large batches from unrelated donor tissues such as bone marrow. In contrast, autologous therapies are manufactures as a single lot from the patient being treated. Here is the latest research on allogenic and autologous therapies.