Adult stem cells exhibit global suppression of RNA polymerase II serine-2 phosphorylation

Stem Cells
Rasmus FreterShin-Ichi Nishikawa

Abstract

Adult stem cells, which are characterized by their capacity for self-renewal and differentiation, participate in tissue homeostasis and response to injury. They are thought to enter a state of relative quiescence, known as reversible cell cycle arrest, but the underlying molecular mechanisms remain poorly characterized. Previous data from our laboratory has shown that housekeeping gene expression is downregulated in melanocyte stem cells (MelSCs), suggesting a global suppression of mRNA transcription. We now show, using antibodies against specific phosphorylated forms of RNA polymerase II (RNApII), that adult MelSCs do not undergo productive mRNA transcription elongation, while RNApII is activated and initialized, ready to synthesize mRNA upon stimulation, and that the RNApII kinase CDK9 is absent in adult MelSCs. Interestingly, other adult stem cells also, including keratinocyte, muscle, spermatogonia, and hematopoietic stem cells, showed a similar absence of RNApII phosphorylation. Although it is difficult to show the functional significance of this observation in vivo, CDK9 inhibition resulted in enhanced survival of cells that are deprived from survival factors. We conclude that the absence of productive mRNA transcription ...Continue Reading

References

Apr 1, 1995·Current Opinion in Genetics & Development·D L Bentley
Apr 1, 1994·Developmental Dynamics : an Official Publication of the American Association of Anatomists·A IrintchevA Wernig
Apr 26, 1994·Proceedings of the National Academy of Sciences of the United States of America·X GrañaA Giordano
Mar 21, 1998·The Journal of Biological Chemistry·M PatturajanJ L Corden
Jan 1, 2000·Journal of Reproduction and Fertility·R AndersonC Wylie
Mar 10, 2000·Science·T ChengD T Scadden
Jan 11, 2001·Journal of Virology·S M FoskettA P Rice
Jun 30, 2001·The Journal of Biological Chemistry·S H Chao, D H Price
Apr 27, 2002·Nature·Emi K NishimuraShin-Ichi Nishikawa
Mar 22, 2003·The Journal of Investigative Dermatology·Carol S TrempusRaymond W Tennant
Jul 16, 2003·Trends in Immunology·Isharat Yusuf, David A Fruman
Sep 5, 2003·Biology of Reproduction·Mito Kanatsu-ShinoharaTakashi Shinohara
Jan 8, 2004·The Journal of Investigative Dermatology·Yaping LiuGeorge Cotsarelis
Mar 24, 2004·Cell·Elaine FuchsGeraldine Guasch
Sep 29, 2004·Journal of Cellular Physiology·Hongbing Liu, Christine H Herrmann
Oct 19, 2004·Genes & Development·Robert J SimsDanny Reinberg
Nov 8, 2005·The Journal of Immunology : Official Journal of the American Association of Immunologists·Renée M MarshallXavier Graña
Nov 30, 2005·Development·Masatake OsawaShin-Ichi Nishikawa
Jan 24, 2006·Developmental Biology·Siu-Shan MakShin-Ichi Nishikawa
Mar 3, 2006·PLoS Biology·Hilary A CollerJames M Roberts
May 25, 2006·Frontiers in Bioscience : a Journal and Virtual Library·Renee M Marshall, Xavier Grana
Jul 17, 2007·Pigment Cell Research·Shin-Ichi Nishikawa, Masatake Osawa
Jul 28, 2007·The Journal of Investigative Dermatology·Saori YonetaniShin-ichi Nishikawa
Nov 13, 2007·Nature Genetics·Julia ZeitlingerRichard A Young
Nov 13, 2007·Nature Genetics·Ginger W MuseKaren Adelman
Jan 19, 2008·Nature Reviews. Genetics·Keith W Orford, David T Scadden
Mar 29, 2008·Cell Stem Cell·Zuzana Tothova, D Gary Gilliland
Nov 5, 2008·Nucleic Acids Research·Judith A BlakeUNKNOWN Mouse Genome Database Group

❮ Previous
Next ❯

Citations

May 24, 2013·Nature Reviews. Molecular Cell Biology·Tom H Cheung, Thomas A Rando
Apr 18, 2012·Proceedings of the National Academy of Sciences of the United States of America·Ballachanda N DevaiahDinah S Singer
Aug 24, 2010·Pigment Cell & Melanoma Research·Keith S Hoek, Colin R Goding
Apr 11, 2012·Pigment Cell & Melanoma Research·Rasmus Freter
Oct 2, 2012·The Journal of Clinical Investigation·Peng HongM A Q Siddiqui
Aug 14, 2010·Epigenetics & Chromatin·Hirofumi FuruhashiWilliam G Kelly
Oct 11, 2014·The International Journal of Biochemistry & Cell Biology·Stuart HodgettsPilar Blancafort
Jun 14, 2012·Nature Communications·Mathilde LatilFabrice Chrétien
Aug 12, 2014·Expert Opinion on Biological Therapy·Ju Hee Lee, David E Fisher
Jul 15, 2011·The Journal of Investigative Dermatology·Satomi Nishikawa-TorikaiShin-Ichi Nishikawa
Nov 5, 2014·Frontiers in Cell and Developmental Biology·So-Ichiro FukadaAkiyoshi Uezumi
Jun 2, 2016·Stem Cells International·Flavia BrunaMartha L Arango-Rodríguez
Jan 1, 2016·Biologie aujourd'hui·Patrick LauretteIrwin Davidson
Mar 25, 2016·Oncotarget·Xin LiFang Li
Aug 2, 2013·Development·Elizabeth Anne BowmanWilliam G Kelly
Feb 12, 2015·Development·Richard L MortE Elizabeth Patton

❮ Previous
Next ❯

Related Concepts

Related Feeds

Blood And Marrow Transplantation

The use of hematopoietic stem cell transplantation or blood and marrow transplantation (bmt) is on the increase worldwide. BMT is used to replace damaged or destroyed bone marrow with healthy bone marrow stem cells. Here is the latest research on bone and marrow transplantation.

Adult Stem Cells

Adult stem cells reside in unique niches that provide vital cues for their survival, self-renewal, and differentiation. They hold great promise for use in tissue repair and regeneration as a novel therapeutic strategies. Here is the latest research.

Cancer Metabolism

In order for cancer cells to maintain rapid, uncontrolled cell proliferation, they must acquire a source of energy. Cancer cells acquire metabolic energy from their surrounding environment and utilize the host cell nutrients to do so. Here is the latest research on cancer metabolism.

Allogenic & Autologous Therapies

Allogenic therapies are generated in large batches from unrelated donor tissues such as bone marrow. In contrast, autologous therapies are manufactures as a single lot from the patient being treated. Here is the latest research on allogenic and autologous therapies.