Adult thymus contains dendritic epidermal T cell precursors that home to the epidermis in irradiated, but not in normal, mice

Cellular Immunology
J HayakawaT Shiohara

Abstract

It was generally assumed that Vgamma5+ fetal thymocytes are the only cells capable of homing to the epidermis. However, we have recently demonstrated that reconstitution of thymectomized irradiated mice with T-cell-depleted bone marrow (BM) cells results in the appearance of donor-type CD8+, TCR-alphabeta+ dendritic epidermal T cells (DETC) phenotypically distinct from their normal gammadelta+ counterparts. In the present study, we found that the epidermis of lethally irradiated, BM-reconstituted (XBM) mice was also repopulated by DETC of host origin as well as donor BM-derived DETC and that the host-derived DETC were rarely detected in the epidermis of athymic BM chimeras (ATXBM). This result indicates that the host-derived DETC are originated from intrathymic radioresistant cells that may share migratory capacities with the fetal thymocytes. We demonstrated that transfer of radioresistant intrathymic precursor cells into ATXBM mice resulted in the appearance of donor-type CD8+, TCR-alphabeta+ DETC. Contrary to our initial expectation, however, transferred normal adult thymocytes also gave rise to donor-type DETC with frequency similar to radioresistant thymocyte-derived DETC. These thymocyte-derived DETC were phenotypically s...Continue Reading

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