Adult Zebrafish (Danio rerio) As a Model for the Study of Corneal Antinociceptive Compounds

Zebrafish
Francisco Ernani Alves MagalhãesAdriana R Campos

Abstract

Zebrafish is an excellent model that can be utilized as an adjunct to current rodent models for studies of eye diseases because the anatomy and ultrastructural characterization of its cornea show much similarity with the human cornea. Therefore, we developed a behavioral model of corneal nociception using the adult zebrafish (Danio rerio). We analyzed the nociceptive effect of hypertonic saline (0.15-5.0 M sodium chloride [NaCl]) applied to the surface of the right or left cornea, on the animals' gender and locomotor activity through the open-field test. The behavioral model of corneal nociception was characterized by the antinociceptive effect of morphine (8.0 or 16 mg/kg; intraperitoneally [i.p.]), an opioid analgesic, and capsazepine, an antagonist of transient receptor potential vanilloid type 1 channels. We also tested whether the corneal antinociceptive effect of morphine could be modulated by naloxone, an opioid antagonist. Finally, we used the light and dark test to assess the anxiolytic effect of hypertonic saline (5.0 M NaCl; 5 μL) applied to the right or left cornea of the animals. As a result, hypertonic saline significantly increased (p < 0.01 vs. control) the corneal nociceptive behavior of adult zebrafish (D. rer...Continue Reading

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Citations

Sep 11, 2019·Journal of Medicinal Food·Larissa Morais Ribeiro da SilvaNágila Maria Pontes Silva Ricardo
Sep 24, 2019·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·Lynne U Sneddon
Jun 24, 2020·Peptides·Richard J Bodnar
Sep 24, 2020·Biochemical and Biophysical Research Communications·Francisca Crislândia Oliveira SilvaHélcio Silva Dos Santos
Apr 27, 2021·Frontiers in Neuroscience·Nils OhnesorgeLars Lewejohann
Jun 21, 2020·International Journal of Biological Macromolecules·Gerlânia de Oliveira LeiteAdriana Rolim Campos
Aug 22, 2021·Journal of Ethnopharmacology·Mahima SharmaDebapriya Garabadu

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