Advanced age results in a diminished endothelial glycocalyx.

American Journal of Physiology. Heart and Circulatory Physiology
Daniel R MachinAnthony J Donato

Abstract

Age-related microvascular dysfunction is well characterized in rodents and humans, but little is known about the properties of the microvascular endothelial glycocalyx in advanced age. We examined the glycocalyx in microvessels of young and old male C57BL6 mice (young: 6.1 ± 0.1 mo vs. old: 24.6 ± 0.2 mo) using intravital microscopy and transmission electron microscopy and in human participants (young: 29 ± 1 yr vs. old: 60 ± 2 yr) using intravital microscopy. Glycocalyx thickness in mesenteric and skeletal muscle microvessels was 51-54% lower in old compared with young mice. We also observed 33% lower glycocalyx thickness in the sublingual microcirculation of humans in advanced age. The perfused boundary region, a marker of glycocalyx barrier function, was also obtained using an automated capture and analysis system. In advanced age, we observed a 10-22% greater perfused boundary region in mice and humans, indicating a more penetrable glycocalyx. Finally, using this automated analysis system, we examined perfused microvascular density and red blood cell (RBC) fraction. Perfused microvascular density is a marker of microvascular function that reflects the length of perfused microvessel segments in a given area; RBC fraction rep...Continue Reading

References

Aug 13, 1999·The American Journal of Physiology·C B Henry, B R Duling
Aug 24, 1999·The Journal of Biological Chemistry·N ItanoK Kimata
Sep 28, 2000·Pflügers Archiv : European journal of physiology·A R PriesP Gaehtgens
Jun 25, 2002·Microcirculation : the Official Journal of the Microcirculatory Society, Inc·T W SecombA R Pries
Sep 18, 2002·American Journal of Physiology. Heart and Circulatory Physiology·A W Mulivor, H H Lipowsky
Dec 5, 2002·Archives of Biochemistry and Biophysics·Hiroshi KamadaEugene J-M A Thonar
Mar 15, 2003·Circulation Research·Bernard M van den BergJos A E Spaan
Mar 22, 2003·American Journal of Physiology. Heart and Circulatory Physiology·John A RussellTimothy I Musch
Jan 6, 2004·American Journal of Physiology. Heart and Circulatory Physiology·A W Mulivor, H H Lipowsky
Mar 16, 2005·Respiratory Physiology & Neurobiology·Brad J BehnkeDavid C Poole
Jul 26, 2005·American Journal of Physiology. Heart and Circulatory Physiology·A R Pries, T W Secomb
Jan 10, 2006·American Journal of Physiology. Heart and Circulatory Physiology·Ivan Rubio-GayossoBrian R Duling
Apr 6, 2006·Journal of Internal Medicine·J M Tarbell, M Y Pahakis
Mar 10, 2007·Journal of Applied Physiology·Pedro CabralesMarcos Intaglietta
May 1, 2007·American Journal of Physiology. Heart and Circulatory Physiology·Yu YaoC Forbes Dewey
Nov 10, 2009·The Annals of Otology, Rhinology, and Laryngology·Tsunehisa OhnoBernard Rousseau
Oct 20, 2011·Annals of Biomedical Engineering·F E Curry, R H Adamson
Jul 17, 2012·Journal of the American Society of Nephrology : JASN·Andrew H J SalmonJanos Peti-Peterdi
Oct 23, 2012·Journal of the American Society of Nephrology : JASN·Carmen A VlahuHans Vink
Jun 6, 2013·Cerebrovascular Diseases·Remy J H MartensJulie Staals
Aug 31, 2013·Microvascular Research·Abele DonatiMervyn Singer
Mar 1, 2014·Journal of Applied Physiology·Bart B L GroenLuc J C van Loon
May 9, 2014·The American Journal of Clinical Nutrition·Daniel R MachinHirofumi Tanaka
Apr 19, 2015·Critical Care : the Official Journal of the Critical Care Forum·Cosimo ChelazziChiara Adembri
May 21, 2016·American Journal of Physiology. Heart and Circulatory Physiology·P Mason McClatcheyJane E B Reusch
May 10, 2017·The Journals of Gerontology. Series A, Biological Sciences and Medical Sciences·May J ReedWilliam A Banks
May 17, 2017·Morphologie : Bulletin De L'Association Des Anatomistes·L ChevalierV Richard

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Citations

Aug 14, 2020·Microcirculation : the Official Journal of the Microcirculatory Society, Inc·Hideshi OkadaHiroyuki Tomita
Sep 29, 2020·Current Heart Failure Reports·Andrew OnegliaC Noel Bairey Merz
Sep 23, 2020·Cardiovascular Engineering and Technology·Sheldon WeinbaumJohn M Tarbell
May 27, 2020·Basic Research in Cardiology·Ilona CuijpersEbba Brakenhielm
Dec 12, 2020·Experimental Biology and Medicine·Aric F LogsdonMichelle A Erickson
Nov 8, 2020·International Journal of Molecular Sciences·Yujiro Kida
Feb 24, 2021·The Ocular Surface·Jeremias G Galletti, Cintia S de Paiva
May 22, 2019·Current Opinion in Pharmacology·Daniel R MachinAnthony J Donato
May 8, 2021·Clinical Science·Anna E StanhewiczGary L Pierce
May 26, 2021·The American Journal of Pathology·Roberto F NicosiaDomenico Ribatti
Jul 31, 2021·PloS One·Elisabeth Buur StougaardPeter Rossing
Aug 10, 2021·Nature Aging·William A BanksMichelle A Erickson
Aug 28, 2021·Biomedicines·Ghassan BkailyDanielle Jacques
Oct 26, 2021·The Journal of Trauma and Acute Care Surgery·Michael J CargeLawrence N Diebel
Dec 5, 2021·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Heidi N du PreezJohnson Lin
Jan 8, 2022·American Journal of Physiology. Heart and Circulatory Physiology·Xiangyu ZhengDaniel R Machin

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Methods Mentioned

BETA
transmission electron microscopy
PCR
electron microscopy
imaging technique

Software Mentioned

SPSS
ImageJ

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