Advanced fluorescence microscopy methods for the real-time study of transcription and chromatin dynamics

Transcription
Paolo Annibale, Enrico Gratton

Abstract

In this contribution we provide an overview of the recent advances allowed by the use of fluorescence microscopy methods in the study of transcriptional processes and their interplay with the chromatin architecture in living cells. Although the use of fluorophores to label nucleic acids dates back at least to about half a century ago, (1) two recent breakthroughs have effectively opened the way to use fluorescence routinely for specific and quantitative probing of chromatin organization and transcriptional activity in living cells: namely, the possibility of labeling first the chromatin loci and then the mRNA synthesized from a gene using fluorescent proteins. In this contribution we focus on methods that can probe rapid dynamic processes by analyzing fast fluorescence fluctuations.

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Citations

Jul 2, 2014·Cell Reports·Adrien SenecalXavier Darzacq
Jun 27, 2015·Trends in Genetics : TIG·Diego I CattoniMarcelo Nollmann
Jun 27, 2015·Biomedical Optics Express·Paolo AnnibaleEnrico Gratton
Mar 28, 2017·Chemical Society Reviews·Yuqiong XiaXiaoyuan Chen

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Methods Mentioned

BETA
fluorescence microscopy
gene array
FCS
Fluorescence Recovery After Photobleaching
Fluorescence
in vivo transcription
fluctuation
light sheet microscopy

Software Mentioned

SPT

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