Abstract
Increased monocytes, particularly the inflammatory subset, are associated with accelerated atherosclerosis in diabetes through thus far incompletely defined mechanisms. The present study tested the hypothesis that advanced glycation end products (AGEs) promote bone marrow monocytes to proliferate and drive them into an inflammatory phenotype. In vivo, AGEs (25 mg/kg i.p. for 7 days) increased proportions of CD115+ monocytes and the inflammatory subset, the CD115+Ly6Chigh cells, in murine bone marrow (flow cytometry analysis (FCM)), and enhanced gene expression of proinflammatory cytokines (IL-1β and TNF-α) but only slightly upregulated mRNA expression of anti-inflammatory cytokine (IL-10) (real-time PCR) in monocytes. In vitro, when the monocytes were treated with different dosages of AGEs (50, 150, and 300 μg/mL), we found that proliferation (CCK8) but not apoptosis (FCM) of the monocytes was induced; the mRNA expressions of proinflammatory cytokines (IL-1β and TNF-α) and GM-CSF were upregulated in a dose-dependent manner while mRNA levels of IL-10 and M-CSF were changed much less in monocytes (real-time PCR). Furthermore, AGEs (300 μg/mL) significantly enhanced the expression of Ki67 in monocytes (immunofluorescence staining ...Continue Reading
References
Jan 15, 2000·Bioscience, Biotechnology, and Biochemistry·T SasakiS Yui
Sep 3, 2004·Cytokine·Magdalena Pertyńska-MarczewskaEwa M Paleolog
Jan 4, 2007·The Journal of Clinical Investigation·Filip K SwirskiMikael J Pittet
Jan 27, 2010·The Biochemical Journal·Robert NewtonMartin W Bergmann
Mar 29, 2011·International Immunopharmacology·Ying HuangQi Xiang Shao
Jun 2, 2011·Molecular Immunology·Kara DoffekJoel Shilyansky
Oct 13, 2011·European Journal of Pharmacology·Yasushi HirasawaTadashi Nagamatsu
Feb 9, 2012·Cardiology in Review·Anand PrasadSotirios Tsimikas
Feb 22, 2012·The Journal of Immunology : Official Journal of the American Association of Immunologists·John R LukensThirumala-Devi Kanneganti
Sep 4, 2012·International Journal of Molecular Sciences·Qiaojing QinYong Gu
Oct 12, 2012·Pflügers Archiv : European journal of physiology·Li-Mei ZhaoXiu-Ling Deng
Feb 28, 2013·Cardiovascular Diabetology·Qiaojing QinYong Gu
May 15, 2013·Cell Metabolism·Prabhakara R NagareddyIra J Goldberg
May 9, 2014·Cell Metabolism·Prabhakara R NagareddyAndrew J Murphy
Dec 9, 2014·Mediators of Inflammation·Christelle Darrieutort-LaffiteBenoit Le Goff
Feb 13, 2015·Molecular and Cellular Biochemistry·Mingfeng MaYingxian Sun
Jun 27, 2015·BioMed Research International·Xian JinChengxing Shen
Dec 22, 2015·Cytokine·Xingxin XuYonggui Wu
Apr 12, 2016·The FEBS Journal·Xian JinChengxing Shen
Apr 28, 2016·Allergology International : Official Journal of the Japanese Society of Allergology·Yasuhiko KogaMasanobu Yamada
Jun 28, 2016·Pharmacological Research : the Official Journal of the Italian Pharmacological Society·Jianxin LiuHua Zhou
Jun 30, 2016·Journal of Leukocyte Biology·Irina Ushach, Albert Zlotnik