Advanced glycation endproducts form during ovalbumin digestion in the presence of fructose: Inhibition by chlorogenic acid

Fitoterapia
Yasmin BainsRussell Caccavello

Abstract

One mechanism by which fructose could exert deleterious effects is through intestinal formation and absorption of pro-inflammatory advanced glycation endproducts via the Maillard reaction. We employed simulated stomach and duodenum digestion of ovalbumin (OVA) to test the hypothesis that advanced glycation endproducts (AGEs) are formed by fructose during simulated digestion of a ubiquitous food protein under model physiological conditions. OVA was subjected to simulated gastric and intestinal digestion using standard models, in presence of fructose or glucose (0-100mM). Peptide fractions were analyzed by fluorescence spectroscopy and intensity at Excitation: λ370nm, Emission: λ 440nm. AGE adducts formed between fructose and OVA, evidenced by the peptide fractions (<5kDa) at times (30min) and concentration ranges (10mM) plausibly found in the intestines, whereas no reaction occurs with glucose. The reaction was inhibited by chlorogenic acid at concentrations compatible with those found in the gut. The reaction was also inhibited by aminoguanidine, a specific antiglycation agent. Our study showed fructose-AGE formation on a ubiquitous dietary protein under model physiological conditions. Our study also suggests ways to decrease t...Continue Reading

Citations

Mar 29, 2018·The British Journal of Nutrition·Luanne R DeChristopher, Katherine L Tucker
Jan 18, 2020·Obesity Reviews : an Official Journal of the International Association for the Study of Obesity·Juliana F TavaresRita C G Alfenas
Sep 18, 2020·Nutrients·Timme van der LugtMisha F Vrolijk
Jul 28, 2019·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Agnieszka PiwowarIzabela Fecka

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