Advanced In vivo Use of CRISPR/Cas9 and Anti-sense DNA Inhibition for Gene Manipulation in the Brain

Frontiers in Genetics
Brandon J WaltersIva B Zovkic

Abstract

Gene editing tools are essential for uncovering how genes mediate normal brain-behavior relationships and contribute to neurodegenerative and neuropsychiatric disorders. Recent progress in gene editing technology now allows neuroscientists unprecedented access to edit the genome efficiently. Although many important tools have been developed, here we focus on approaches that allow for rapid gene editing in the adult nervous system, particularly CRISPR/Cas9 and anti-sense nucleotide-based techniques. CRISPR/Cas9 is a flexible gene editing tool, allowing the genome to be manipulated in diverse ways. For instance, CRISPR/Cas9 has been successfully used to knockout genes, knock-in mutations, overexpress or inhibit gene activity, and provide scaffolding for recruiting specific epigenetic regulators to individual genes and gene regions. Moreover, the CRISPR/Cas9 system may be modified to target multiple genes at one time, affording simultaneous inhibition and overexpression of distinct genetic targets. Although many of the more advanced applications of CRISPR/Cas9 have not been applied to the nervous system, the toolbox is widely accessible, such that it is poised to help advance neuroscience. Anti-sense nucleotide-based technologies ...Continue Reading

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Citations

May 21, 2016·Frontiers in Molecular Neuroscience·Weili YangXiao-Jiang Li
Apr 11, 2017·Progress in Neuro-psychopharmacology & Biological Psychiatry·Sen YanXiao-Jiang Li
Dec 5, 2017·Policy Insights From the Behavioral and Brain Sciences·Tania L Roth
Jan 5, 2018·Molecular Psychiatry·África FloresRaül Andero
Mar 1, 2019·Molecular Psychiatry·Gal Richter-LevinMathias V Schmidt
May 24, 2017·Scientific Reports·Emmanouil MetzakopianAllan Bradley
Oct 12, 2017·ACS Chemical Biology·Ross C Wilson, Luke A Gilbert

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Methods Mentioned

BETA
gene knockdown
transgenic
gene knockout
gene knockouts
histone acetylation
transfection
SMA

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