Advanced paternal age directly impacts mouse embryonic placental imprinting

PloS One
Michelle M DenommeMandy G Katz-Jaffe

Abstract

The placental epigenome plays a critical role in regulating mammalian growth and development. Alterations to placental methylation, often observed at imprinted genes, can lead to adverse pregnancy complications such as intrauterine growth restriction and preterm birth. Similar associations have been observed in offspring derived from advanced paternal age fathers. As parental age at time of conception continues to rise, the impact of advanced paternal age on these reproductive outcomes is a growing concern, but limited information is available on the molecular mechanisms affected in utero. This longitudinal murine research study thus investigated the impact of paternal aging on genomic imprinting in viable F1 embryonic portions of the placentas derived from the same paternal males when they were young (4-6 months) and when they aged (11-15 months). The use of a controlled outbred mouse model enabled analysis of offspring throughout the natural lifetime of the same paternal males and excluded confounding factors like female age or infertility. Firstly, paternal age significantly impacted embryonic placental weight, fetal weight and length. Targeted bisulfite sequencing was utilized to examine imprinted methylation at the Kcnq1ot...Continue Reading

References

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Citations

Nov 11, 2020·Journal of Assisted Reproduction and Genetics·Stanislav A VasilyevIgor N Lebedev
Feb 13, 2021·American Journal of Reproductive Immunology : AJRI·Sushama RokadeVineeta Bal

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Methods Mentioned

BETA
PCR

Software Mentioned

REST
Genomatix
QUMA
BISMA
MatInspector
Relative Expression Software Tool ( REST

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