Advanced quantitative proteomics to evaluate molecular effects of low-molecular-weight hyaluronic acid in human dermal fibroblasts.

Journal of Pharmaceutical and Biomedical Analysis
Silvia RadrezzaAlfonsina D'Amato

Abstract

Hyaluronic acid (HA) is physiologically synthesized by several human cells types but it is also a widespread ingredient of commercial products, from pharmaceuticals to cosmetics. Despite its extended use, the precise intra- and extra-cellular effects of HA at low-molecular-weight (LWM-HA) are currently unclear. At this regard, the aim of this study is to in-depth identify and quantify proteome's changes in normal human dermal fibroblasts after 24 h treatment with 0.125, 0.25 and 0.50 % LMW-HA (20-50 kDa) respectively, vs controls. To do this, a label-free quantitative proteomic approach based on high-resolution mass spectrometry was used. Overall, 2328 proteins were identified of which 39 significantly altered by 0.125 %, 149 by 0.25 % and 496 by 0.50 % LMW-HA. Protein networking studies indicated that the biological effects involve the enhancement of intracellular activity at all concentrations, as well as the extracellular matrix reorganization, proteoglycans and collagen biosynthesis. Moreover, the cell's wellness was confirmed, although mild inflammatory and immune responses were induced at the highest concentration. The more complete comprehension of intra- and extra-cellular effects of LMW-HA here provided by an advanced ...Continue Reading

Citations

Feb 17, 2021·The Journal of the American Academy of Orthopaedic Surgeons·Zahra RezasoltaniFatemeh Vashaei
Apr 30, 2021·Carbohydrate Polymers·Zhi-Yuan YaoJin-Song Shi
Aug 28, 2021·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Silvia RadrezzaAlfonsina D'Amato

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