Advances in Intravital Non-Linear Optical Imaging of the Central Nervous System in Rodents

Brain Plasticity
Geneviève RougonFranck Debarbieux

Abstract

Purpose of review: Highly coordinated cellular interactions occur in the healthy or pathologic adult rodent central nervous system (CNS). Until recently, technical challenges have restricted the analysis of these events to largely static modes of study such as immuno-fluorescence and electron microscopy on fixed tissues. The development of intravital imaging with subcellular resolution is required to probe the dynamics of these events in their natural context, the living brain. Recent findings: This review focuses on the recently developed live non-linear optical imaging modalities, the core principles involved, the identified technical challenges that limit their use and the scope of their applications. We highlight some practical applications for these modalities with a specific attention given to Experimental Autoimmune Encephalomyelitis (EAE), a rodent model of a chronic inflammatory disease of the CNS characterized by the formation of disseminated demyelinating lesions accompanied by axonal degeneration. Summary: We conclude that label-free nonlinear optical imaging combined to two photon imaging will continue to contribute richly to comprehend brain function and pathogenesis and to develop effective therapeutic strategies.

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Citations

Oct 28, 2017·Brain Imaging and Behavior·Sandra AlbaneseMarcello Mancini

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Methods Mentioned

BETA
imaging techniques
fluorescence microscopy
transgenic
confocal microscopy
light-scattering
imaging technique
FRET
fluorescence recovery after photobleaching
nonlinear microscopy
optical microscopy

Software Mentioned

Brainbow

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